Anti-IL1 Receptor I/IL-1R-1 antibody (ab154524)
Key features and details
- Rabbit polyclonal to IL1 Receptor I/IL-1R-1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL1 Receptor I/IL-1R-1 antibody
See all IL1 Receptor I/IL-1R-1 primary antibodies -
Description
Rabbit polyclonal to IL1 Receptor I/IL-1R-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human IL1 Receptor I/IL-1R-1 aa 505-569 (C terminal).
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2 and Raji cell lysate; Human breast carcinoma tissue.
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General notes
Previously labelled as IL1 Receptor I.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab154524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 65 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0). |
Target
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Function
Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2. -
Sequence similarities
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3554 Human
- Omim: 147810 Human
- SwissProt: P14778 Human
- Unigene: 701982 Human
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Alternative names
- Antigen CD121a antibody
- CD121 antigen-like family member A antibody
- CD121a antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL1 Receptor I/IL-1R-1 antibody (ab154524)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue, labeling IL-1 Receptor I/IL-1R-1 using ab154524 at a 1/500 dilution.
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Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody (ab154524)Anti-IL1 Receptor I/IL-1R-1 antibody (ab154524) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 65 kDa
7.5% SDS PAGE
Protocols
References (1)
ab154524 has been referenced in 1 publication.
- Lefley D et al. Development of clinically relevant in vivo metastasis models using human bone discs and breast cancer patient-derived xenografts. Breast Cancer Res 21:130 (2019). PubMed: 31783893
