Key features and details
- Rabbit polyclonal to IL1 Receptor I/IL-1R-1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IL1 Receptor I/IL-1R-1 antibody
See all IL1 Receptor I/IL-1R-1 primary antibodies
DescriptionRabbit polyclonal to IL1 Receptor I/IL-1R-1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human IL1 Receptor I/IL-1R-1 aa 505-569 (C terminal).
- 293T, A431, H1299, HeLaS3, HepG2 and Raji cell lysate; Human breast carcinoma tissue.
Previously labelled as IL1 Receptor I.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 65 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).|
FunctionReceptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2.
Sequence similaritiesBelongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain.
- Information by UniProt
- Antigen CD121a antibody
- CD121 antigen-like family member A antibody
- CD121a antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue, labeling IL-1 Receptor I/IL-1R-1 using ab154524 at a 1/500 dilution.
Anti-IL1 Receptor I/IL-1R-1 antibody (ab154524) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 65 kDa
7.5% SDS PAGE
ab154524 has been referenced in 1 publication.
- Lefley D et al. Development of clinically relevant in vivo metastasis models using human bone discs and breast cancer patient-derived xenografts. Breast Cancer Res 21:130 (2019). PubMed: 31783893