Anti-IL1 Receptor I/IL-1R-1 antibody (ab154524)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-IL1 Receptor I/IL-1R-1 antibody
See all IL1 Receptor I/IL-1R-1 primary antibodies -
Description
Rabbit polyclonal to IL1 Receptor I/IL-1R-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human IL1 Receptor I/IL-1R-1 aa 505-569 (C terminal).
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2 and Raji cell lysate; Human breast carcinoma tissue.
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General notes
Previously labelled as IL1 Receptor I.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab154524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 65 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0). |
Target
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Function
Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2. -
Sequence similarities
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3554 Human
- Omim: 147810 Human
- SwissProt: P14778 Human
- Unigene: 701982 Human
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Alternative names
- Antigen CD121a antibody
- CD121 antigen-like family member A antibody
- CD121a antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL1 Receptor I/IL-1R-1 antibody (ab154524)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue, labeling IL-1 Receptor I/IL-1R-1 using ab154524 at a 1/500 dilution.
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Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody (ab154524)Anti-IL1 Receptor I/IL-1R-1 antibody (ab154524) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 65 kDa
7.5% SDS PAGE
References
ab154524 has not yet been referenced specifically in any publications.
