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    il1-receptor-iil-1r-1-antibody-ab154524.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Anti-IL1 Receptor I/IL-1R-1 antibody (ab154524)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL1 Receptor I/IL-1R-1 antibody (ab154524)
  • Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody (ab154524)

Key features and details

  • Rabbit polyclonal to IL1 Receptor I/IL-1R-1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Recombinant Human IL1 Receptor I/IL-1R-1 protein (Fc Chimera) (ab83575)

View more associated products

Overview

  • Product name

    Anti-IL1 Receptor I/IL-1R-1 antibody
    See all IL1 Receptor I/IL-1R-1 primary antibodies
  • Description

    Rabbit polyclonal to IL1 Receptor I/IL-1R-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human IL1 Receptor I/IL-1R-1 aa 505-569 (C terminal).

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2 and Raji cell lysate; Human breast carcinoma tissue.
  • General notes

    Previously labelled as IL1 Receptor I. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • A431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human IL1 Receptor I/IL-1R-1 protein (Fc Chimera) (ab83575)

Applications

Our Abpromise guarantee covers the use of ab154524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 65 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).

Target

  • Function

    Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2.
  • Sequence similarities

    Belongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P14778 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3554 Human
    • Omim: 147810 Human
    • SwissProt: P14778 Human
    • Unigene: 701982 Human
    • Alternative names

      • Antigen CD121a antibody
      • CD121 antigen-like family member A antibody
      • CD121a antibody
      • CD121a antigen antibody
      • D2S1473 antibody
      • IL 1 antibody
      • IL 1R 1 antibody
      • IL 1R alpha antibody
      • IL 1RT1 antibody
      • IL-1R-1 antibody
      • IL-1R-alpha antibody
      • IL-1RT-1 antibody
      • IL-1RT1 antibody
      • IL1 inhibitor antibody
      • IL1 Receptor I antibody
      • IL1R antibody
      • IL1R1 antibody
      • IL1R1_HUMAN antibody
      • IL1RA antibody
      • IL1RN antibody
      • IL1RT1 antibody
      • Interleukin 1 receptor alpha type 1 antibody
      • Interleukin 1 receptor antagonist protein (IRAP) antibody
      • Interleukin 1 receptor type I antibody
      • Interleukin receptor 1 antibody
      • Interleukin-1 receptor alpha antibody
      • Interleukin-1 receptor type 1 antibody
      • Interleukin-1 receptor type I antibody
      • p80 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL1 Receptor I/IL-1R-1 antibody (ab154524)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL1 Receptor I/IL-1R-1 antibody (ab154524)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue, labeling IL-1 Receptor I/IL-1R-1 using ab154524 at a 1/500 dilution.

    • Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody (ab154524)
      Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody (ab154524)
      Anti-IL1 Receptor I/IL-1R-1 antibody (ab154524) at 1/1000 dilution + Raji whole cell lysate at 30 µg

      Predicted band size: 65 kDa



      7.5% SDS PAGE

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab154524? Please let us know so that we can cite the reference in this datasheet.

    ab154524 has been referenced in 1 publication.

    • Lefley D  et al. Development of clinically relevant in vivo metastasis models using human bone discs and breast cancer patient-derived xenografts. Breast Cancer Res 21:130 (2019). PubMed: 31783893

    Customer reviews and Q&As

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    Please use the links above to contact us or submit feedback about this product.

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