Recombinant
RabMAb

Recombinant Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] (ab229051)

Overview

  • Product name

    Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36]
    See all IL1 Receptor I/IL-1R-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR22198-36] to IL1 Receptor I/IL-1R-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse IL1 Receptor I/IL-1R-1 aa 1-350. The exact sequence is proprietary.
    Database link: P13504

  • Positive control

    • WB: NIH/3T3, EL4.IL2 and bEnd.3 whole cell lysates; Mouse lung lysate.
  • General notes

    Previously labelled as IL1 Receptor I. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab229051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 80, 65 kDa (predicted molecular weight: 65 kDa).

Target

  • Function

    Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2.
  • Sequence similarities

    Belongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antigen CD121a antibody
    • CD121 antigen-like family member A antibody
    • CD121a antibody
    • CD121a antigen antibody
    • D2S1473 antibody
    • IL 1 antibody
    • IL 1R 1 antibody
    • IL 1R alpha antibody
    • IL 1RT1 antibody
    • IL-1R-1 antibody
    • IL-1R-alpha antibody
    • IL-1RT-1 antibody
    • IL-1RT1 antibody
    • IL1 inhibitor antibody
    • IL1 Receptor I antibody
    • IL1R antibody
    • IL1R1 antibody
    • IL1R1_HUMAN antibody
    • IL1RA antibody
    • IL1RN antibody
    • IL1RT1 antibody
    • Interleukin 1 receptor alpha type 1 antibody
    • Interleukin 1 receptor antagonist protein (IRAP) antibody
    • Interleukin 1 receptor type I antibody
    • Interleukin receptor 1 antibody
    • Interleukin-1 receptor alpha antibody
    • Interleukin-1 receptor type 1 antibody
    • Interleukin-1 receptor type I antibody
    • p80 antibody
    see all

Images

  • All lanes : Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] (ab229051) at 1/1000 dilution

    Lane 1 : Mouse lung lysate
    Lane 2 : EL4.IL-2 (mouse lymphoma T lymphocyte cell line) whole cell lysate
    Lane 3 : bEnd.3 (mouse brain endothelioma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 65 kDa
    Observed band size: 65,80 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane 1: 48 seconds, Lane 2: 15 seconds, Lane 3: 114 seconds.

    The 80 kDa band represents the membrane form, while the 65 kDa band is the soluble form (PMID: 17324958, PMID: 28450382).

  • All lanes : Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] (ab229051) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 2 : B16-F10 (mouse melanoma mixture of spindle-shaped and epithelial-like cells) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 65 kDa
    Observed band size: 65,80 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST .

    Exposure times.

    Lane 1: 37 seconds, Lane 2: 3 minutes.

    The 80 kDa band represents the membrane form, while the 65 kDa band is the soluble form (PMID: 17324958, PMID: 28450382).

    Negative control: B16-F10 (PMID: 29445180).

References

ab229051 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229051.
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