Recombinant Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] - BSA and Azide free (ab238457)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22198-36] to IL1 Receptor I/IL-1R-1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] - BSA and Azide free
See all IL1 Receptor I/IL-1R-1 primary antibodies -
Description
Rabbit monoclonal [EPR22198-36] to IL1 Receptor I/IL-1R-1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: NIH/3T3, EL4.IL2 and bEnd.3 whole cell lysate.
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General notes
ab238457 is the carrier-free version of ab229051.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22198-36 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab238457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 80, 65 kDa (predicted molecular weight: 65 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 80, 65 kDa (predicted molecular weight: 65 kDa). |
Target
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Function
Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2. -
Sequence similarities
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16177 Mouse
- SwissProt: P13504 Mouse
- Unigene: 896 Mouse
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Alternative names
- Antigen CD121a antibody
- CD121 antigen-like family member A antibody
- CD121a antibody
see all
Images
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All lanes : Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] (ab229051) at 1/1000 dilution
Lane 1 : EL4.IL-2 (Mouse lymphoma T lymphocyte) cell lysate
Lane 2 : bEnd.3 (Mouse brain endothelioma ) cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229051).
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All lanes : Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] (ab229051) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : EL4.IL-2 (mouse lymphoma T lymphocyte cell line) whole cell lysate
Lane 3 : bEnd.3 (mouse brain endothelioma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 65 kDa
Observed band size: 65,80 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229051).
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lane 1: 48 seconds, Lane 2: 15 seconds, Lane 3: 114 seconds.
The 80 kDa band represents the membrane form, while the 65 kDa band is the soluble form (PMID: 17324958, PMID: 28450382).
This blot was developed using a high sensitivity ECL substrate.
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All lanes : Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] (ab229051) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : B16-F10 (mouse melanoma mixture of spindle-shaped and epithelial-like cells) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 65 kDa
Observed band size: 65,80 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST .
Exposure times.
Lane 1: 37 seconds, Lane 2: 3 minutes.
The 80 kDa band represents the membrane form, while the 65 kDa band is the soluble form (PMID: 17324958, PMID: 28450382).
Negative control: B16-F10 (PMID: 29445180).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229051).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab238457 has been referenced in 1 publication.
- Liskiewicz A et al. Methamphetamine-associated cognitive decline is attenuated by neutralizing IL-1 signaling. Brain Behav Immun N/A:N/A (2019). PubMed: 30885840