Anti-IL11RA antibody [EPR5446] (ab125015)


  • Product name
    Anti-IL11RA antibody [EPR5446]
    See all IL11RA primary antibodies
  • Description
    Rabbit monoclonal [EPR5446] to IL11RA
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IL11RA aa 350-450. The exact sequence is proprietary.

  • Positive control
    • 293T, Saos2, A673, PC3, HuT78, K562, and TF1 cell lysates; Human kidney tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab125015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Receptor for interleukin-11. The receptor systems for IL6, LIF, OSM, CNTF, IL11 and CT1 can utilize IL6ST for initiating signal transmission. The IL11/IL11RA/IL6ST complex may be involved in the control of proliferation and/or differentiation of skeletogenic progenitor or other mesenchymal cells.
    • Tissue specificity
      Expressed in a number of cell lines, including the myelogenous leukemia cell line K562, the megakaryocytic leukemia cell line Mo7E, the erythroleukemia cell line TF1, and the osteosarcoma cell lines, MG-63 and Saos-2. Also expressed in normal and malignant prostate epithelial cell lines. Expression levels are increased in prostate carcinoma.
    • Sequence similarities
      Belongs to the type I cytokine receptor family. Type 3 subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • CRSDA antibody
      • hCG_2011440 antibody
      • I11RA_HUMAN antibody
      • IL-11 receptor subunit alpha antibody
      • IL-11R subunit alpha antibody
      • IL-11R-alpha antibody
      • IL-11RA antibody
      • IL11RA antibody
      • Interleukin 11 receptor alpha antibody
      • Interleukin 11 receptor alpha chain antibody
      • Interleukin-11 receptor subunit alpha antibody
      • MGC2146 antibody
      see all


    • Overlay histogram showing K562 cells stained with ab125015 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab125015, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-IL11RA antibody [EPR5446] (ab125015) at 1/1000 dilution

      Lane 1 : 293T lysate
      Lane 2 : Saos2 lysate
      Lane 3 : A673 lysate
      Lane 4 : PC3 lysate
      Lane 5 : Hut78 lysate
      Lane 6 : K562 lysate
      Lane 7 : TF1 lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 45 kDa
      Observed band size: 45 kDa

    • ab125015 at 1/500 staining IL11RA in paraffin embedded Human Kidney tissue by immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    ab125015 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your reply.

    Indeed, while this product is tested and guaranteed to work in Flow Cytometry, as the epitope is cytoplasmic, cells will need to be permeablized.

    I hope this information is helpful to you. Please do n...

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    Thank you for contacting us.

    The exact immunogen information for this product is considered proprietary and as such I am unable to share the sequence with you. I can however let you know that the immunogen used to create this product did c...

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    Thank you for contacting us.

    For the WB image containing various cell line lysates, 10 ug of protein was loaded.
    5% non fat milk in TBST was used for blocking for 1 hr, Primary antibodu was used at a dilution of 1/1000 in 5%BSA/TBST over...

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