Anti-IL-1RA antibody (ab175392)
Key features and details
- Rabbit polyclonal to IL-1RA
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-IL-1RA antibody
See all IL-1RA primary antibodies -
Description
Rabbit polyclonal to IL-1RA -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human IL-1RA.
Database link: P18510 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100.
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WB |
1/1000. Predicted molecular weight: 20 kDa.
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Notes |
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ICC/IF
1/100. |
WB
1/1000. Predicted molecular weight: 20 kDa. |
Target
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Function
Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. -
Tissue specificity
The intracellular form of IL1RN is predominantly expressed in epithelial cells. -
Involvement in disease
Microvascular complications of diabetes 4
Interleukin 1 receptor antagonist deficiency -
Cellular localization
Cytoplasm and Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3557 Human
- Omim: 147679 Human
- SwissProt: P18510 Human
- Unigene: 81134 Human
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Alternative names
- DIRA antibody
- F630041P17Rik antibody
- ICIL 1RA antibody
see all
Images
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Anti-IL-1RA antibody (ab175392) at 1/1000 dilution + A431 cells at 25 µg
Secondary
HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 20 kDa
Exposure time: 90 secondsBlocking buffer: 3% nonfat dry milk in TBST
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Immunofluorescence analysis of human skin cancer using ab175392 at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of A431 cells using ab175392 at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of HepG2 cells using ab175392 at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab175392 has been referenced in 3 publications.
- Li S et al. Spontaneous immunomodulation and regulation of angiogenesis and osteogenesis by Sr/Cu-borosilicate glass (BSG) bone cement to repair critical bone defects. Bioact Mater 23:101-117 (2023). PubMed: 36406252
- Gajawada P et al. Myocardial Accumulations of Reg3A, Reg3? and Oncostatin M Are Associated with the Formation of Granulomata in Patients with Cardiac Sarcoidosis. Int J Mol Sci 22:N/A (2021). PubMed: 33923774
- Jurga AM et al. Lipopolysaccharide from Rhodobacter sphaeroides (TLR4 antagonist) attenuates hypersensitivity and modulates nociceptive factors. Pharm Biol 56:275-286 (2018). PubMed: 29656686