Anti-IL2 Receptor alpha antibody [MEM-181] (ab8235)


  • Product name
    Anti-IL2 Receptor alpha antibody [MEM-181]
    See all IL2 Receptor alpha primary antibodies
  • Description
    Mouse monoclonal [MEM-181] to IL2 Receptor alpha
  • Host species
  • Specificity
    CD25 antigen (human).
  • Tested applications
    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Tissue/ cell preparation (Human). (PHA-activated peripheral blood leucocytes).

  • Epitope



Our Abpromise guarantee covers the use of ab8235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 2 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


ICC/IF 1/1000.


  • Function
    Receptor for interleukin-2.
  • Involvement in disease
    Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • Sequence similarities
    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Interleukin 2 receptor alpha chain antibody
    • CD25 antibody
    • CD25 antigen antibody
    • IDDM10 antibody
    • IL 2 receptor alpha subunit antibody
    • IL-2 receptor subunit alpha antibody
    • IL-2-RA antibody
    • IL-2R subunit alpha antibody
    • IL2 RA antibody
    • IL2-RA antibody
    • IL2R antibody
    • IL2R, alpha chain antibody
    • IL2RA antibody
    • IL2RA_HUMAN antibody
    • IMD41 antibody
    • Interleukin 2 receptor alpha antibody
    • Interleukin 2 receptor antibody
    • Interleukin-2 receptor subunit alpha antibody
    • p55 antibody
    • t-cell growth factor receptor antibody
    • TAC antibody
    • TAC antigen antibody
    • TCGFR antibody
    see all


  • Overlay histogram showing Jurkat cells stained with ab8235 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8235, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed Jurkat cells used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.


This product has been referenced in:
  • Brusés JL Cell surface localization of a3ß4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility. PLoS One 8:e62435 (2013). ICC/IF ; Human . Read more (PubMed: 23626818) »
  • Nielsen MS  et al. Different motifs regulate trafficking of SorCS1 isoforms. Traffic 9:980-94 (2008). ICC/IF ; Mouse . Read more (PubMed: 18315530) »

See all 2 Publications for this product

Customer reviews and Q&As

The antibody ab8232 is unconjugated and so you would need to use a secondary antibody for detection. If you tell me what techniques you are using I would be happy to make some suggestions about suitable secondary antibodies. Please let me know if ther...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Human Cell (HEK293 tranfected with chimeric receptor construct)
HEK293 tranfected with chimeric receptor construct
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10%

Abcam user community

Verified customer

Submitted Jul 17 2008

I can confirm that this product does not required pemeabilisation when it was tested in FACS as the recognized epitope is extracellular. Please do not hesitate to contact us if you require further information.

Thank you for your enquiry. I ran a search for a neutralising antibody directed against the IL2 Receptor alpha and unfortunately could find any results. This does not mean that the ab8235 will not work in blocking IL2, it just means that we have not te...

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Thank you for your message. We are very sorry but there was a discrepancy on our datasheet. We would like to confirm that this antibody has not been tested and characterised for Western blot application. Thank you for your feedback; it is very ...

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Our datasheet currently states that the antibody has not yet been tested in WB and I apologise if it previously did; I have checked with our source and they confirmed they have not tested it yet. Should you wish a refund can you please give me your...

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