Key features and details
- Mouse monoclonal [1G11C6] to IL2 Receptor beta/p75
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-IL2 Receptor beta/p75 antibody [1G11C6]
See all IL2 Receptor beta/p75 primary antibodies
DescriptionMouse monoclonal [1G11C6] to IL2 Receptor beta/p75
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey
Recombinant fragment corresponding to Human IL2 Receptor beta/p75 aa 27-240. Expressed in E.coli.
AVNGTSQFTCFYNSRANISCVWSQDGALQDTSCQVHAWPDRRRWNQTCEL LPVSQASWACNLILGAPDSQKLTTVDIVTLRVLCREGVRWRVMAIQDFKP FENLRLMAPISLQVVHVETHRCNISWEISQASHYFERHLEFEARTLSPGH TWEEAPLLTLKQKQEWICLETLTPDTQYEFQVRVKPLQGEFTTWSPWSQP LAFRTKPAALGKDT
Database link: P14784
- WB: Recombinant human IL2 Receptor beta/p75 (aa 27-240) protein; IL2 Receptor beta/p75 (aa 27-240)-hIgG-Fc-transfected HEK-293 cell lysate. Flow cyt: HL-60 and Jurkat cells.
This product was previously labelled as IL2 Receptor beta
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab233792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000.|
|Flow Cyt||1/200 - 1/400.|
FunctionReceptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 1 fibronectin type-III domain.
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
- Information by UniProt
- CD122 antibody
- CD122 antigen antibody
- High affinity IL 2 receptor beta subunit antibody
Anti-IL2 Receptor beta/p75 antibody [1G11C6] (ab233792) at 1/500 dilution + Recombinant human IL2 Receptor beta/p75 (aa 27-240) protein
Expected MW is 51kDa.
All lanes : Anti-IL2 Receptor beta/p75 antibody [1G11C6] (ab233792) at 1/500 dilution
Lane 1 : Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : IL2 Receptor beta/p75 (aa 27-240)-hIgG-Fc-transfected HEK-293 cell lysate
Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling IL2 Receptor beta/p75 with ab233792 at 1/200 dilution (green) compared with a negative control (red).
Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling IL2 Receptor beta/p75 with ab233792 at 1/200 dilution (green) compared with a negative control (red).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233792 has not yet been referenced specifically in any publications.