Anti-IL2 Receptor beta/p75 antibody [1G11C6] (ab233792)
![Western blot - Anti-IL2 Receptor beta/p75 antibody [1G11C6] (ab233792)](https://www.abcam.com/ps/products/233/ab233792/Images/ab233792-307500-anti-il2-receptor-beta-antibody-1g11c6-western-blot.jpg "Western blot - Anti-IL2 Receptor beta/p75 antibody [1G11C6] (ab233792)")
Key features and details
- Mouse monoclonal [1G11C6] to IL2 Receptor beta/p75
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-IL2 Receptor beta/p75 antibody [1G11C6]
See all IL2 Receptor beta/p75 primary antibodies -
Description
Mouse monoclonal [1G11C6] to IL2 Receptor beta/p75 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey
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Immunogen
Recombinant fragment corresponding to Human IL2 Receptor beta/p75 aa 1-250. Expressed in E.coli.
Database link: P14784 -
Positive control
- WB: Recombinant human IL2 Receptor beta/p75 (aa 27-240) protein; IL2 Receptor beta/p75 (aa 27-240)-hIgG-Fc-transfected HEK-293 cell lysate. Flow cyt: HL-60 and Jurkat cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1G11C6 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/2000.
|
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| Flow Cyt |
1/200 - 1/400.
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| Notes |
|---|
|
WB
1/500 - 1/2000. |
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Flow Cyt
1/200 - 1/400. |
Target
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Function
Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 1 fibronectin type-III domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3560 Human
- Omim: 146710 Human
- SwissProt: Q38J84 Chimpanzee
- SwissProt: Q38J85 Cynomolgus monkey
- SwissProt: P14784 Human
- Unigene: 474787 Human
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Alternative names
- CD122 antibody
- CD122 antigen antibody
- High affinity IL 2 receptor beta subunit antibody
see all
Images
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Western blot - Anti-IL2 Receptor beta/p75 antibody [1G11C6] (ab233792)Anti-IL2 Receptor beta/p75 antibody [1G11C6] (ab233792) at 1/500 dilution + Recombinant human IL2 Receptor beta/p75 (aa 27-240) protein
Expected MW is 51kDa.
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![Western blot - Anti-IL2 Receptor beta/p75 antibody [1G11C6] (ab233792)](https://www.abcam.com/ps/products/233/ab233792/Images/ab233792-307501-anti-il2-receptor-beta-antibody-1g11c6-western-blot.jpg)
Western blot - Anti-IL2 Receptor beta/p75 antibody [1G11C6] (ab233792)All lanes : Anti-IL2 Receptor beta/p75 antibody [1G11C6] (ab233792) at 1/500 dilution
Lane 1 : Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : IL2 Receptor beta/p75 (aa 27-240)-hIgG-Fc-transfected HEK-293 cell lysate -
![Flow Cytometry - Anti-IL2 Receptor beta/p75 antibody [1G11C6] (ab233792)](https://www.abcam.com/ps/products/233/ab233792/Images/ab233792-307502-anti-il2-receptor-beta-antibody-1g11c6-flow-cytometry.jpg)
Flow Cytometry - Anti-IL2 Receptor beta/p75 antibody [1G11C6] (ab233792)Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling IL2 Receptor beta/p75 with ab233792 at 1/200 dilution (green) compared with a negative control (red).
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![Flow Cytometry - Anti-IL2 Receptor beta/p75 antibody [1G11C6] (ab233792)](https://www.abcam.com/ps/products/233/ab233792/Images/ab233792-307503-anti-il2-receptor-beta-antibody-1g11c6-flow-cytometry.jpg)
Flow Cytometry - Anti-IL2 Receptor beta/p75 antibody [1G11C6] (ab233792)Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling IL2 Receptor beta/p75 with ab233792 at 1/200 dilution (green) compared with a negative control (red).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab233792 has not yet been referenced specifically in any publications.
