Overview

  • Product name

    Anti-IL2 Receptor beta/p75 antibody
    See all IL2 Receptor beta/p75 primary antibodies
  • Description

    Rabbit polyclonal to IL2 Receptor beta/p75
  • Host species

    Rabbit
  • Specificity

    ab61195 detects endogenous levels of total IL2 Receptor beta/p75 protein.

  • Tested applications

    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human IL2 Receptor beta/p75.

  • Positive control

    • Human lung carcinoma tissue and COS7 cell extracts.
  • General notes

     This product was previously labelled as IL2 Receptor beta

     

Properties

Applications

Our Abpromise guarantee covers the use of ab61195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
ELISA 1/20000.

Target

  • Function

    Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 4 subfamily.
    Contains 1 fibronectin type-III domain.
  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD122 antibody
    • CD122 antigen antibody
    • High affinity IL 2 receptor beta subunit antibody
    • High affinity IL 2 receptor subunit beta antibody
    • High affinity IL-2 receptor subunit beta antibody
    • IL-2 receptor subunit beta antibody
    • IL-2R subunit beta antibody
    • IL-2RB antibody
    • IL15RB antibody
    • IL2 receptor antibody
    • IL2RB antibody
    • IL2RB_HUMAN antibody
    • Interleukin 2 receptor beta antibody
    • Interleukin-2 receptor subunit beta antibody
    • P70 75 antibody
    • P70-75 antibody
    • P7075 antibody
    • p75 antibody
    see all

Images

  • ab61195 at 1/50 dilution staining IL2 Receptor beta/p75 in human lung carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence (left) or presence (right) of the immunising peptide.

  • All lanes : Anti-IL2 Receptor beta/p75 antibody (ab61195) at 1/500 dilution

    Lane 1 : COS7 cell extracts
    Lane 2 : COS7 cell extracts with immunising peptide

    Predicted band size: 61 kDa
    Observed band size: 61 kDa

References

This product has been referenced in:

  • Iskandar R  et al. Expression of pericardial fluid T-cells and related inflammatory cytokines in patients with chronic heart failure. Exp Ther Med 13:1850-1858 (2017). Read more (PubMed: 28565777) »
  • Lu ZH  et al. CD25 is a novel marker of hepatic bile canaliculus. Int J Surg Pathol 20:455-61 (2012). ICC/IF ; Human . Read more (PubMed: 22576476) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrat pH 6
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde

Frau Dr. Martina Dettwiler

Verified customer

Submitted Dec 06 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spleen)
Permeabilization
No
Specification
Spleen
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 20% · Temperature: 37°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Jul 28 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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