Recombinant Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] (ab263906)
![Immunocytochemistry/ Immunofluorescence - Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] (ab263906)](https://www.abcam.com/ps/products/263/ab263906/Images/ab263906-2-anti-il2-receptor-beta-p75-antibody-epr22892-2-immunocytochemistry-pbmc-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] (ab263906)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22892-2] to IL2 Receptor beta/p75
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IL2 Receptor beta/p75 antibody [EPR22892-2]
See all IL2 Receptor beta/p75 primary antibodies -
Description
Rabbit monoclonal [EPR22892-2] to IL2 Receptor beta/p75 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Human PBMC's;Flow Cyt: Human PBMC's.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22892-2 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab263906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/500.
|
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| ICC/IF |
1/50.
|
| Notes |
|---|
|
Flow Cyt
1/500. |
|
ICC/IF
1/50.
|
Target
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Function
Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 1 fibronectin type-III domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3560 Human
- Omim: 146710 Human
- SwissProt: P14784 Human
- Unigene: 474787 Human
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Alternative names
- CD122 antibody
- CD122 antigen antibody
- High affinity IL 2 receptor beta subunit antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] (ab263906)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PBMC cells labelling IL2 Receptor beta/p75 with ab263906 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in a subset of Human PBMC cells. is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
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![Flow Cytometry - Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] (ab263906)](https://www.abcam.com/ps/products/263/ab263906/Images/ab263906-1-anti-il2-receptor-beta-p75-antibody-epr22892-2-flowcytometry-pbmc-human.jpg)
Flow Cytometry - Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] (ab263906)Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling IL2 Receptor beta/p75 with ab263906 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) isotype control (left) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab263906 (Right). Then stained with anti-CD56 conjugated to BV421. IL2 Receptor beta/CD122 is constitutively expressed primarily by NK cells (PMID: 24055329). Gated on viable cells.
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![Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] (ab263906)](https://www.abcam.com/ps/products/263/ab263906/Images/ab263906-5-benefits-of-recombinant-antibodies.png)
Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] (ab263906)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab263906 has not yet been referenced specifically in any publications.
