Product nameAnti-IL2 Receptor beta/p75 antibody [EPR22892-2]
See all IL2 Receptor beta/p75 primary antibodies
DescriptionRabbit monoclonal [EPR22892-2] to IL2 Receptor beta/p75
Tested applicationsSuitable for: Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P or WB
Species reactivityReacts with: Human
Recombinant fragment within Human IL2 Receptor beta/p75 aa 1-250. The exact sequence is proprietary.
Database link: P14784
- ICC/IF: Human PBMC's; FC: Human PBMC's.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab263906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionReceptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 1 fibronectin type-III domain.
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
- Information by UniProt
- CD122 antibody
- CD122 antigen antibody
- High affinity IL 2 receptor beta subunit antibody
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PBMC cells labelling IL2 Receptor beta/p75 with ab263906 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in a subset of Human PBMC cells. is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling IL2 Receptor beta/p75 with ab263906 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) isotype control (left) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab263906 (Right). Then stained with anti-CD56 conjugated to BV421. IL2 Receptor beta/CD122 is constitutively expressed primarily by NK cells (PMID: 24055329). Gated on viable cells.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab263906 has not yet been referenced specifically in any publications.