Recombinant
RabMAb

Recombinant Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] (ab263906)

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Overview

  • Product name

    Anti-IL2 Receptor beta/p75 antibody [EPR22892-2]
    See all IL2 Receptor beta/p75 primary antibodies

  • Description

    Rabbit monoclonal [EPR22892-2] to IL2 Receptor beta/p75

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human IL2 Receptor beta/p75 aa 1-250. The exact sequence is proprietary.
    Database link: P14784

  • Positive control

    • ICC/IF: Human PBMC's; FC: Human PBMC's.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab263906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.
ICC/IF 1/50.

 

 
  • Application notes
    Is unsuitable for IHC-P or WB.

    • Target

    • Function

      Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.

    • Sequence similarities

      Belongs to the type I cytokine receptor family. Type 4 subfamily.
      Contains 1 fibronectin type-III domain.

    • Domain

      The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
      The box 1 motif is required for JAK interaction and/or activation.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD122 antibody
        • CD122 antigen antibody
        • High affinity IL 2 receptor beta subunit antibody
        • High affinity IL 2 receptor subunit beta antibody
        • High affinity IL-2 receptor subunit beta antibody
        • IL-2 receptor subunit beta antibody
        • IL-2R subunit beta antibody
        • IL-2RB antibody
        • IL15RB antibody
        • IL2 receptor antibody
        • IL2RB antibody
        • IL2RB_HUMAN antibody
        • Interleukin 2 receptor beta antibody
        • Interleukin-2 receptor subunit beta antibody
        • P70 75 antibody
        • P70-75 antibody
        • P7075 antibody
        • p75 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] (ab263906)
        Immunocytochemistry/ Immunofluorescence - Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] (ab263906)

        Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PBMC cells labelling IL2 Receptor beta/p75 with ab263906 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in a subset of Human PBMC cells. is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

        Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

      • Flow Cytometry - Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] (ab263906)
        Flow Cytometry - Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] (ab263906)

        Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling IL2 Receptor beta/p75 with ab263906 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) isotype control (left) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

        Cells were stained with rabbit IgG (Left) or ab263906 (Right). Then stained with anti-CD56 conjugated to BV421. IL2 Receptor beta/CD122 is constitutively expressed primarily by NK cells (PMID: 24055329). Gated on viable cells.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References

      Publishing research using ab263906? Please let us know so that we can cite the reference in this datasheet.

      ab263906 has not yet been referenced specifically in any publications.

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