Recombinant Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (ab271040)
![Western blot - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (ab271040)](https://www.abcam.com/ps/products/271/ab271040/Images/ab271040-465178-anti-il2-receptor-betap75-antibody-epr2433629-western-blot-lymphoma.jpg "Western blot - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (ab271040)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24336-29] to IL2 Receptor beta/p75
- Suitable for: IP, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-IL2 Receptor beta/p75 antibody [EPR24336-29]
See all IL2 Receptor beta/p75 primary antibodies -
Description
Rabbit monoclonal [EPR24336-29] to IL2 Receptor beta/p75 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details
Unsuitable for: Flow Cyt (Intra) or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HDLM-2 whole cell lysate. HDLM-2 treated with PNGase F whole cell lysate. IHC-P: Human spleen, endometrium, and non-Hodgkin's lymphoma tissues. IP: HDLM-2 cell whole cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24336-29 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271040 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/30.
|
|
| WB |
1/1000. Predicted molecular weight: 61 kDa.
|
|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
IP
1/30. |
|
WB
1/1000. Predicted molecular weight: 61 kDa. |
|
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Function
Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 1 fibronectin type-III domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 3560 Human
- Omim: 146710 Human
- SwissProt: P14784 Human
- Unigene: 474787 Human
-
Alternative names
- CD122 antibody
- CD122 antigen antibody
- High affinity IL 2 receptor beta subunit antibody
see all
Images
-
Western blot - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (ab271040)All lanes : Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (ab271040) at 1/1000 dilution
Lane 1 : HDLM-2 (human Hodgkin lymphoma) whole cell lysate
Lane 2 : Daudi (human burkitts lymphoma lymphoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 61 kDa
Observed band size: 75/37 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The antibody recognizes the full length and the intracellular fragment with molecular weights of 75KDa and 37KDa. (PMID: 20495002).
Negative control: Daudi (PMID:25486870).
Exposure time: 59 seconds.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (ab271040)](https://www.abcam.com/ps/products/271/ab271040/Images/ab271040-1-anti-il2-receptor-betap75-antibody-epr2433629-immunohistochemistry-human-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (ab271040)Immunohistochemical analysis of paraffin-embedded human spleen tissue labelling IL2 Receptor beta/p75 with ab271040 at 1/100 (4.78 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on scattered immune cells of human spleen is observed. The section was incubated with ab271040 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (ab271040)](https://www.abcam.com/ps/products/271/ab271040/Images/ab271040-4-anti-il2-receptor-betap75-antibody-epr2433629-immunohistochemistry-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (ab271040)Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling IL2 Receptor beta/p75 with ab271040 at 1/100 (4.78 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in human cerebrum. The section was incubated with ab271040 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunoprecipitation - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (ab271040)](https://www.abcam.com/ps/products/271/ab271040/Images/ab271040-5-anti-il2-receptor-betap75-antibody-epr2433629-immunoprecipitation-hdlm2.jpg)
Immunoprecipitation - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (ab271040)IL2 Receptor beta/p75 was immunoprecipitated from 0.35 mg HDLM-2 (human Hodgkin lymphoma) whole cell lysate 10 µg with ab271040 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271040 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HDLM-2 whole cell lysate 10 µg
Lane 2: ab271040 IP in HDLM-2 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab271040 in HDLM-2 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 84 seconds.
-
![Western blot - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (ab271040)](https://www.abcam.com/ps/products/271/ab271040/Images/ab271040-465179-anti-il2-receptor-betap75-antibody-epr2433629-western-blot-lymphoma1.jpg)
Western blot - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (ab271040)All lanes : Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (ab271040) at 1/1000 dilution
Lane 1 : HDLM-2 (human Hodgkin lymphoma) whole cell lysate
Lane 2 : HDLM-2 whole cell lysate treated with PNGase F
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 61 kDa
Observed band size: 75/37 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 20495002).
Exposure time: 92 seconds.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (ab271040)](https://www.abcam.com/ps/products/271/ab271040/Images/ab271040-2-anti-il2-receptor-betap75-antibody-epr2433629-immunohistochemistry-human-endometrium.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (ab271040)Immunohistochemical analysis of paraffin-embedded human endometrium tissue labelling IL2 Receptor beta/p75 with ab271040 at 1/100 (4.78 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on scattered immune cells of human endometrium is observed. The section was incubated with ab271040 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (ab271040)](https://www.abcam.com/ps/products/271/ab271040/Images/ab271040-3-anti-il2-receptor-betap75-antibody-epr2433629-immunohistochemistry-human-nonhodgkins-lymphoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (ab271040)Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma tissue labelling IL2 Receptor beta/p75 with ab271040 at 1/100 (4.78 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on human non-Hodgkin's lymphoma. The section was incubated with ab271040 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (ab271040)](https://www.abcam.com/ps/products/271/ab271040/Images/ab271040-6-Benefits-of-Recombinant-antibodies.jpg)
Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (ab271040)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab271040 has not yet been referenced specifically in any publications.