The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml.
ab193252 has been found to neutralize by 50% the biological effect of 0.1 ng/mL mouse IL23 in an assay of IL17 secretion by mouse splenocytes. The G23-8 antibody has no effect on the bioactivity of mouse IL12 p70.
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
Use at an assay dependent concentration.
Use a concentration of 1 - 4 µg/ml.
Use as the capture antibody in an IL23 ELISA assay (in conjunction with biotinylated C17.8 antibody).
Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).