Recombinant Anti-IL-3 antibody [EPR7964] (ab167159)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7964] to IL-3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-IL-3 antibody [EPR7964]
See all IL-3 primary antibodies -
Description
Rabbit monoclonal [EPR7964] to IL-3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human IL-3. The exact sequence is proprietary.
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Positive control
- Jurkat cells. Human recombinant IL3 protein.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7964 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab167159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 17 kDa. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. -
Tissue specificity
Activated T-cells, mast cells, natural killer cells. -
Sequence similarities
Belongs to the IL-3 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3562 Human
- Entrez Gene: 16187 Mouse
- Omim: 147740 Human
- SwissProt: P08700 Human
- SwissProt: P01586 Mouse
- Unigene: 694 Human
- Unigene: 983 Mouse
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Alternative names
- Colony stimulating factor multiple antibody
- Hematopoietic growth factor antibody
- IL 3 antibody
see all
Images
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Anti-IL-3 antibody [EPR7964] (ab167159) at 1.23 µg/ml + Mouse IL-3 recombinant protein at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 17 kDa
Observed band size: 17 kDa
Blocking/diluting Buffer and concentration: 5% NFDM /TBST -
Immunocytochemical analysis of Jurkat cells labeling IL3 with ab167159 at a 1/100 dilution.
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Anti-IL-3 antibody [EPR7964] (ab167159) at 1/1000 dilution + Human IL3 recombinant protein (10 ng)
Predicted band size: 17 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab167159 has been referenced in 1 publication.
- Zhao DD et al. Evidence for a role of geranylgeranylation in renal angiomyolipoma and renal epithelioid angiomyolipoma. Oncol Lett 17:1523-1530 (2019). PubMed: 30675208