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Western blot - Anti-IL36 alpha antibody (ab116279)
All lanes : Anti-IL36 alpha antibody (ab116279) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue Lane 2 : Brain (Human) Tissue Lysate - fetal normal tissue Lane 3 : Brain (Human) Tissue Lysate - adult normal tissue with Immunising peptide at 1 µg/ml Lane 4 : Brain (Human) Tissue Lysate - fetal normal tissue with Immunising peptide at 1 µg/ml
Lysates/proteins at 25 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab116279 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.