Overview

  • Product name

    Anti-IL36 alpha/IL-1F6 antibody - C-terminal
    See all IL36 alpha/IL-1F6 primary antibodies
  • Description

    Rabbit polyclonal to IL36 alpha/IL-1F6 - C-terminal
  • Host species

    Rabbit
  • Specificity

    ab180909 will not cross-react with IL36 beta or IL36 gamma.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human IL36 alpha/IL-1F6 (C terminal). (11 amino acid peptide; NP_055255).
    Database link: Q9UHA7

  • Positive control

    • MCF7 cell lysate; Human brain tissue; mouse brain tissue.
  • General notes

    Previously labelled as IL36 alpha. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).
IHC-P Use a concentration of 5 - 20 µg/ml.

Target

  • Tissue specificity

    Expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines.
  • Sequence similarities

    Belongs to the IL-1 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • FIL1 antibody
    • FIL1 epsilon antibody
    • FIL1(EPSILON) antibody
    • FIL1E antibody
    • IL 1 epsilon antibody
    • IL 1F6 antibody
    • IL 1H1 antibody
    • IL-1 epsilon antibody
    • IL-1F6 antibody
    • IL1E antibody
    • IL1F6 antibody
    • IL1F6_HUMAN antibody
    • IL1RP2 antibody
    • IL36 alpha antibody
    • IL36A antibody
    • Interleukin 1 epsilon antibody
    • Interleukin 1 family member 6 (epsilon) antibody
    • Interleukin 1 family member 6 antibody
    • Interleukin 36 alpha antibody
    • Interleukin-1 epsilon antibody
    • Interleukin-1 family member 6 antibody
    • MGC129552 antibody
    • MGC129553 antibody
    • MGC151479 antibody
    • MGC151481 antibody
    • OTTMUSP00000012798 antibody
    • RP23-176J12.4 antibody
    see all

Images

  • All lanes : Anti-IL36 alpha/IL-1F6 antibody - C-terminal (ab180909) at 1 µg/ml

    Lane 1 : MCF7 cell lysate
    Lane 2 : MCF7 cell lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 18 kDa
    Observed band size: 16 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling IL36 alpha / IL-1F6 with ab180909 at 20 µg/ml.

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling IL36 alpha / IL-1F6 with ab180909 at 5 µg/ml.

References

This product has been referenced in:

  • Frew JW  et al. A systematic review and critical evaluation of inflammatory cytokine associations in hidradenitis suppurativa. F1000Res 7:1930 (2018). Read more (PubMed: 30828428) »
  • Russell SE  et al. IL-36a expression is elevated in ulcerative colitis and promotes colonic inflammation. Mucosal Immunol 9:1193-204 (2016). Read more (PubMed: 26813344) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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