Recombinant Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] (ab269271)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23089-87] to IL36 alpha/IL-1F6
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87]
See all IL36 alpha/IL-1F6 primary antibodies -
Description
Rabbit monoclonal [EPR23089-87] to IL36 alpha/IL-1F6 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC or IHC-Fr -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: Human tonsil tissue lysate. IHC-P: Human psoriatic skin. Human tonsil tissue. Human colon carcinoma tissue. WB: Human tonsil tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23089-87 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Immunohistochemistry kits
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/30.
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Predicted molecular weight: 18 kDa.
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Notes |
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IP
1/30. |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Predicted molecular weight: 18 kDa. |
Target
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Tissue specificity
Expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines. -
Sequence similarities
Belongs to the IL-1 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 27179 Human
- Omim: 605509 Human
- SwissProt: Q9UHA7 Human
- Unigene: 278910 Human
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Alternative names
- FIL1 antibody
- FIL1 epsilon antibody
- FIL1(EPSILON) antibody
see all
Images
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Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] (ab269271) at 1/1000 dilution + Human tonsil tissue lysate at 20 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 18 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 mins.
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Immunohistochemical analysis of paraffin-embedded human psoriatic skin tissue labeling IL36 alpha/IL-1F6 with ab269271 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human psoriatic skin is observed (PMID: 28043870). The section was incubated with ab269271 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded human skin tissue labeling IL36 alpha/IL-1F6 with ab269271 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Negative control: No staining on human healthy skin (PMID: 28043870).
The section was incubated with ab269271 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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IL36 alpha/IL-1F6 was immunoprecipitated from 0.35 mg human tonsil tissue lysate with ab269271 at 1/30. Western blot was performed on the immunoprecipitate using ab269271 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Human tonsil tissue lysate.
Lane 2: ab269271 IP in human tonsil tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269271 in human tonsil tissue lysate.
Blocking and dilution buffer and concentration/ 5% NFDM/TBST.
Exposure time: 24 secs.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IL36 alpha/IL-1F6 with ab269271 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on tonsil epithelium is observed. The section was incubated with ab269271 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling IL36 alpha/IL-1F6 with ab269271 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human colon carcinoma tissue is observed (PMID: 30452921). The section was incubated with ab269271 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab269271 has not yet been referenced specifically in any publications.