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Rabbit polyclonal to IL36 beta
Predicted to work with:
Macaque monkey, Gorilla
Synthetic peptide corresponding to Human IL36 beta aa 50-150 conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: Q9NZH7
This antibody gave a positive signal in Human Tonsil tissue lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 19 kDa).
Expression at low levels in tonsil, bone marrow, heart, placenta, lung, testis and colon but not in other tissues or in any hematopoietic cell lines.
Belongs to the IL-1 family.
Information by UniProt
Family of interleukin 1 eta antibody
FIL1 (ETA) antibody
Western blot - Anti-IL36 beta antibody (ab116280)
All lanes :
Anti-IL36 beta antibody (ab116280) at 1 µg/ml (Milk blocking 3%)
Lane 1 :
Tonsil (Human) Tissue Lysate
Lane 2 :
Tonsil (Human) Tissue Lysate with Immunising peptide at 1 µg/ml
Lysates/proteins at 25 µg per lane.
Secondary All lanes :
Donkey Anti-Rabbit IgG H&L preadsorbed (
) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
20 kDa (
why is the actual band size different from the predicted?
Additional bands at:
37 kDa (possible non-specific binding)
This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab116280 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
has not yet been referenced specifically in any publications.
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