Key features and details
- Rabbit polyclonal to IL36 beta/IL-1F8 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IL36 beta/IL-1F8 antibody - C-terminal
DescriptionRabbit polyclonal to IL36 beta/IL-1F8 - C-terminal
Specificityab180890 will only detect the longer isoform of IL36 beta / IL-1F8. ab180890 will not cross-react with IL36 alpha or IL36 gamma.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human IL36 beta/IL-1F8 (C terminal). (16 amino acid peptide; NP_055253).
Database link: Q9NZH7
- A549 cell lysate; Human lung tissue.
Previously labelled as IL36 beta.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab180890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 19 kDa).|
|IHC-P||Use a concentration of 2.5 - 20 µg/ml.|
Tissue specificityExpression at low levels in tonsil, bone marrow, heart, placenta, lung, testis and colon but not in other tissues or in any hematopoietic cell lines.
Sequence similaritiesBelongs to the IL-1 family.
- Information by UniProt
- 2310043N20Rik antibody
- Family of interleukin 1 eta antibody
- FIL1 (ETA) antibody
All lanes : Anti-IL36 beta/IL-1F8 antibody - C-terminal (ab180890) at 1 µg/ml
Lane 1 : A549 cell lysate
Lane 2 : A549 cell lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 19 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling IL36 beta / IL-1F8 with ab180890 at 2.5 μg/ml.
Immunohistochemical analysis of Human lung tissue labeling IL36 beta / IL-1F8 with ab180890 at 20 µg/ml.
ab180890 has been referenced in 2 publications.
- Frew JW et al. A systematic review and critical evaluation of inflammatory cytokine associations in hidradenitis suppurativa. F1000Res 7:1930 (2018). PubMed: 30828428
- Frew JW et al. A systematic review and critical evaluation of immunohistochemical associations in hidradenitis suppurativa. F1000Res 7:1923 (2018). PubMed: 31281635