Product nameAnti-IL36 gamma/IL-1F9 antibody [EPR23333-107] - BSA and Azide free
See all IL36 gamma/IL-1F9 primary antibodies
DescriptionRabbit monoclonal [EPR23333-107] to IL36 gamma/IL-1F9 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Recombinant fragment within Human IL36 gamma/IL-1F9 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q9NZH8
- WB: HaCaT (treated with 20ng/ml IL-1beta and 50ng/ml TNFalpha for 20 hours,) whole cell lysate; His-tagged recombinant human IL36G protein (aa18-169).
ab273095 is the carrier-free version of ab272731. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab273095 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.??
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab273095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 18 kDa (predicted molecular weight: 19 kDa).|
FunctionFunction as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. Could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1), that is present in epithelial barriers and takes part in local inflammatory response.
Tissue specificityHighly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus. In skin is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Up-regulated in lesional psoriasis skin.
Sequence similaritiesBelongs to the IL-1 family.
- Information by UniProt
- IL 1 epsilon antibody
- IL 1 related protein 2 antibody
- IL 1(EPSILON) antibody
All lanes : Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731) at 1/1000 dilution
Lane 1 : HaCaT (human skin keratinocyte), whole cell lysate
Lane 2 : HaCaT treated with 20ng/ml IL-1beta and 50ng/ml TNF-alpha for 20 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 19 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression of IL36 gamma/IL-1F9 is induced by IL-1beta and TNF-alpha treatment in HaCaT (PMID: 23095752).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 81 seconds.
These data were developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab272731).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab273095 has not yet been referenced specifically in any publications.