Overview

  • Product name

  • Description

    Rabbit polyclonal to IL37
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human IL37 aa 45-208.

  • Positive control

    • A431 whole cell lysate; 293T and Raji cell lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 20% Glycerol, 1.21% Tris
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.

Target

  • Function

    Suppressor of innate inflammatory and immune responses involved in curbing excessive inflammation. This function requires SMAD3. Suppresses, or reduces, proinflammatory cytokine production, including IL1A and IL6, as well as CCL12, CSF1, CSF2, CXCL13, IL1B, IL23A and IL1RN, but spares anti-inflammatory cytokines. Inhibits dendritic cell activation.
  • Tissue specificity

    In general, low constitutive expression, if any, in healthy tissues; high expression in inflammatory counterparts, including in synovial tissues from individuals with active rheumatoid arthritis. Isoform A, isoform B and isoform C are expressed in testis, colon, placenta, lung and lymph node. Isoform D and isoform E were found only in testis and bone marrow. Whereas only isoform A is found in brain, only isoform B in kidney and only isoform C in heart.
  • Sequence similarities

    Belongs to the IL-1 family.
  • Post-translational
    modifications

    Proteolytically converted to the mature form by CASP1.
  • Cellular localization

    Cytoplasm > cytosol. Nucleus. Secreted. Stimulation with IL1B leads to colocalization with SMAD3 mostly in perinuclear regions. Only the CASP1-cleaved mature form translocates into the nucleus upon LPS stimulation.
  • Information by UniProt
  • Database links

  • Alternative names

    • FIL1 antibody
    • FIL1 zeta antibody
    • FIL1(ZETA) antibody
    • FIL1Z antibody
    • IL 1 zeta antibody
    • IL 1F7 antibody
    • IL 1F7b (IL 1H4, IL 1H, IL 1RP1) antibody
    • IL 1H4 antibody
    • IL 1RP1 antibody
    • IL 1X antibody
    • IL 1X protein antibody
    • IL-1 zeta antibody
    • IL-1F7 antibody
    • IL-1H antibody
    • IL-1H4 antibody
    • IL-1RP1 antibody
    • IL-1X antibody
    • IL-37 antibody
    • IL1F7 (canonical product IL 1F7b) antibody
    • IL1F7 antibody
    • IL1F7_HUMAN antibody
    • IL1H4 antibody
    • IL1RP1 antibody
    • Interleukin 1 family member 7 antibody
    • interleukin 1 family, member 7 (zeta) antibody
    • Interleukin 1 homolog 4 antibody
    • Interleukin 1 related protein antibody
    • Interleukin 1 superfamily z antibody
    • Interleukin 1 zeta antibody
    • Interleukin 37 antibody
    • Interleukin-1 family member 7 antibody
    • Interleukin-1 homolog 4 antibody
    • Interleukin-1 zeta antibody
    • Interleukin-1-related protein antibody
    • Interleukin-23 antibody
    see all

Images

  • Anti-IL37 antibody (ab101376) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 24 kDa



    12% SDS-PAGE

References

This product has been referenced in:

  • Ge G  et al. Interleukin-37 suppresses tumor growth through inhibition of angiogenesis in non-small cell lung cancer. J Exp Clin Cancer Res 35:13 (2016). WB . Read more (PubMed: 26791086) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question

Thank you so much for your reply. The size of IL37 isoform b should be 25kDa, How about the size of isoform a, c, d and e?


























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Dear Sir/Madam



Thank you for contacting us. Based on the immunogen sequence the antibody should detect all 5 isoforms.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Best regards,
Heather

Heather Allen
Scientific Support Specialist
Abcam Inc.
www.abcam.com


Your original inquiry to Abcam:


Hello,
I want to order IL37 antibody (ab101376) from your company. I just wonder about one thing. IL37 has five isoforms a-e. Which isoform can be detected with this antibody. Actually, I want to detect Il37 isoform b.

I am looking forward to hearing from you. Thanks.

Yunbo

University of western Ontario

Canada








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Read More
Answer

Hello! The MW of the isoforms of IL37 are as follows:

Isoform A 21 kD

Isoform C 19 kD

Isofrm D 22 kD

Isofrm E 17 kD

For your convenience we do have links on our datasheet that can connect you to resources like the Swiss-Prot database to provide you with protein information.

Please let me know if you have any further questions!

Read More

Answer

Thank you for contacting us. Based on the immunogen sequence the antibody should detect all 5 isoforms.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

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