Overview

  • Product name

  • Description

    Goat polyclonal to IL3RA/CD123
  • Host species

    Goat
  • Specificity

    ab205365 is expected to recognize both reported isoforms.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human IL3RA/CD123 aa 146-157 (internal sequence). NCBI Accession No. NP_002174.1 and NP_001254642.1.
    Sequence:

    RQQYECLHYKTD


    Database link: P26951

  • Positive control

    • Jurkat and MOLT4 cell lysates.
  • General notes

    Previously labelled as IL3RA. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab205365 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab205365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 43 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    This is a receptor for interleukin-3.
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 5 subfamily.
  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD123 antibody
    • CD123 antigen antibody
    • hIL 3Ra antibody
    • hIL-3Ra antibody
    • hIL3Ra antibody
    • IL 3 receptor alpha SP2 isoform antibody
    • IL 3R alpha antibody
    • IL-3 receptor subunit alpha antibody
    • IL-3R subunit alpha antibody
    • IL-3R-alpha antibody
    • IL-3RA antibody
    • IL3 Receptor alpha antibody
    • IL3R antibody
    • Il3ra antibody
    • IL3RA_HUMAN antibody
    • IL3RAX antibody
    • IL3RAY antibody
    • IL3RX antibody
    • IL3RY antibody
    • Interleukin 3 receptor alpha chain antibody
    • Interleukin 3 receptor, alpha (low affinity) antibody
    • Interleukin 3 receptor, alpha antibody
    • Interleukin-3 Receptor alpha antibody
    • Interleukin-3 receptor subunit alpha antibody
    • Interleukin3 receptor antibody
    • Interleukin3 receptor, Y-chromosomal antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human colon tissue sections, staining IL3RA / CD123 with ab205365 at 5µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

  • All lanes : Anti-IL3RA/CD123 antibody (ab205365) at 2 µg/ml

    Lane 1 : Jurkat cell lysate (in RIPA buffer)
    Lane 2 : MOLT4 cell lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 43 kDa



    Primary incubation was 1 hour.

References

ab205365 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab205365.
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