Recombinant Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free (ab269871)
Overview
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Product name
Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free
See all IL3RA/CD123 primary antibodies -
Description
Rabbit monoclonal [EPR23188-72] to IL3RA/CD123 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF,IHC-Fr or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human IL3RA/CD123 aa 1-350. The exact sequence is proprietary.
Database link: P26951 -
Positive control
- WB: HDLM-2, TF-1, Human tonsil and Human tonsil treated with PNGase F lysates. IHC-P: Human tonsil tissue.
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General notes
ab269871 is the carrier-free version of ab222103. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab269871 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.??
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23188-72 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab269871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 60, 43 kDa (predicted molecular weight: 43 kDa). | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
This is a receptor for interleukin-3. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 5 subfamily. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3563 Human
- Omim: 308385 Human
- Omim: 430000 Human
- SwissProt: P26951 Human
- Unigene: 632790 Human
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Alternative names
- CD123 antibody
- CD123 antigen antibody
- hIL 3Ra antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free (ab269871)](https://www.abcam.com/ps/products/269/ab269871/Images/ab269871-1-anti-il3ra-cd123-antibody-epr23188-72-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free (ab269871)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling IL3RA/CD123 with ab222103 at 1/500 dilution (0.99 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil (PMID: 27340692). The section was incubated with ab222103 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222103).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References
ab269871 has not yet been referenced specifically in any publications.
