Recombinant Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free (ab269871)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free (ab269871)](https://www.abcam.com/ps/products/269/ab269871/Images/ab269871-1-anti-il3ra-cd123-antibody-epr23188-72-immunohistochemistry-tonsil-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free (ab269871)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23188-72] to IL3RA/CD123 - BSA and Azide free
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
-
Product name
Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free
See all IL3RA/CD123 primary antibodies -
Description
Rabbit monoclonal [EPR23188-72] to IL3RA/CD123 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, IHC-Pmore details
Unsuitable for: ICC/IF,IHC-Fr or IP -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HDLM-2, TF-1, Human tonsil and Human tonsil treated with PNGase F lysates. IHC-P: Human tonsil tissue.
-
General notes
ab269871 is the carrier-free version of ab222103.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23188-72 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 60, 43 kDa (predicted molecular weight: 43 kDa).
|
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Detects a band of approximately 60, 43 kDa (predicted molecular weight: 43 kDa). |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Function
This is a receptor for interleukin-3. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 5 subfamily. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 3563 Human
- Omim: 308385 Human
- Omim: 430000 Human
- SwissProt: P26951 Human
- Unigene: 632790 Human
-
Alternative names
- CD123 antibody
- CD123 antigen antibody
- hIL 3Ra antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free (ab269871)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling IL3RA/CD123 with ab222103 at 1/500 dilution (0.99 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil (PMID: 27340692). The section was incubated with ab222103 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222103).
-
![ELISA - Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free (ab269871)](https://www.abcam.com/ps/products/269/ab269871/Images/ab269871-2-anti-il3racd123-antibody-epr23188-72-elisa-human-il3racd123.jpg)
ELISA - Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free (ab269871)This data was developed using ab222103, the same antibody clone in a different buffer formulation.
ELISA analysis of Human IL3RA/CD123 recombinant protein at 1000 ng/mL with ab222103. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody. -
![Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free (ab269871)](https://www.abcam.com/ps/products/269/ab269871/Images/ab269871-3-benefits-of-recombinant-antibodies.png)
Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free (ab269871)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab269871 has not yet been referenced specifically in any publications.
