Anti-IL-3RB (phospho Y593) antibody (ab53695)
Key features and details
- Rabbit polyclonal to IL-3RB (phospho Y593)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL-3RB (phospho Y593) antibody
See all IL-3RB primary antibodies -
Description
Rabbit polyclonal to IL-3RB (phospho Y593) -
Host species
Rabbit -
Specificity
ab53695 detects endogenous levels of IL3RB only when phosphorylated at tyrosine 593. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human IL-3RB aa 550-650 (phospho Y593).
Database link: P32927 -
Positive control
- Human brain tissue and RAW264.7 cell extracts treated with G-CSF (25ng/ml, 15mins).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab53695 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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WB |
1/500 - 1/1000. Predicted molecular weight: 97 kDa.
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Notes |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Predicted molecular weight: 97 kDa. |
Target
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Function
High affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 2 fibronectin type-III domains. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1439 Human
- Entrez Gene: 12983 Mouse
- Omim: 138981 Human
- SwissProt: P32927 Human
- SwissProt: P26955 Mouse
- Unigene: 592192 Human
- Unigene: 235324 Mouse
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Alternative names
- Beta-C antibody
- CD131 antibody
- CDw131 antibody
see all
Images
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ab53695 at 1/50 dilution staining IL3RB in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
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All lanes : Anti-IL-3RB (phospho Y593) antibody (ab53695)
Lane 1 : HepG2 cells treated with EGF (200ng/ml, 15 minutes)
Lane 2 : HepG2 cells treated with EGF (200ng/ml, 15 minutes) with blocking phosphopeptide
Predicted band size: 97 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab53695 has not yet been referenced specifically in any publications.