• Product name
  • Description
    Rabbit polyclonal to IL4I1
  • Host species
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human IL4I1.

    (Peptide available as ab19824.)

  • Positive control
    • WB: Human testis tissue lysate. ICC/IF: HeLa cells.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab18524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 19436310
WB Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).


  • Relevance
    IL-4-induced gene-1 (Il4i1 or Fig1) is expressed in primarily immune tissues and genetically maps to a region of susceptibility to autoimmune disease. The predicted Il4i1 protein (IL4I1) sequence is most similar to apoptosis-inducing protein and Apoxin I, which are both l-amino acid oxidases.
  • Cellular localization
  • Database links
  • Alternative names
    • FIG 1 antibody
    • Fig 1 protein antibody
    • FIG1 antibody
    • Fig1 protein antibody
    • HCG2043070 antibody
    • hFIG 1 antibody
    • hFIG1 antibody
    • IL4 induced protein 1 antibody
    • Interleukin 4 induced 1 antibody
    • Interleukin 4 induced protein 1 antibody
    • Interleukin four induced 1 antibody
    • L amino acid oxidase antibody
    • LAAO antibody
    • LAO antibody
    • Protein Fig 1 antibody
    • Protein Fig1 antibody
    see all


  • Anti-IL4I1 antibody (ab18524) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 20 µg

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 63 kDa
    Observed band size: 65 kDa (why is the actual band size different from the predicted?)

    Exposure time: 3 minutes


    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab18524 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • ICC/IF image of ab18524 stained HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were fixed in paraformaldehyde for 10 minutes, permabilized in TBS-T (20 minutes) and incubated with the antibody (ab18524, 5 µg/ml) for 1 hour at room temperature. 1% BSA / 10% normal goat serum / 0.3M glycine was used to quench auto-fluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).


This product has been referenced in:
  • Marquet J  et al. Dichotomy between factors inducing the immunosuppressive enzyme IL-4-induced gene 1 (IL4I1) in B lymphocytes and mononuclear phagocytes. Eur J Immunol 40:2557-68 (2010). Read more (PubMed: 20683900) »
  • Carbonnelle-Puscian A  et al. The novel immunosuppressive enzyme IL4I1 is expressed by neoplastic cells of several B-cell lymphomas and by tumor-associated macrophages. Leukemia 23:952-60 (2009). IHC-P ; Human . Read more (PubMed: 19436310) »

See all 3 Publications for this product

Customer reviews and Q&As

Western blot
Mouse Cell lysate - whole cell (BMDM)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 10 2016

Thank you for contacting us and sorry for the delay in getting back to you.

I have looked into the reason why the Anti-IL4I1 antibody (ab18524) has been unavailable for some time now. Unfortunately, we have been trying to produce new batches ...

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