Recombinant Anti-IL-6 antibody [EPR21710] (ab229381)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21710] to IL-6
- Suitable for: WB, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-IL-6 antibody [EPR21710]
See all IL-6 primary antibodies -
Description
Rabbit monoclonal [EPR21710] to IL-6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: RAW 264.7 treated with LPS and BFA, whole cell lysate. IP: RAW 264.7 treated with LPS and BFA, whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21710 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
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IP |
1/30.
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Notes |
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WB
1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). |
IP
1/30. |
Target
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Function
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
Involvement in disease
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 16193 Mouse
- SwissProt: P08505 Mouse
- Unigene: 1019 Mouse
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Alternative names
- Interleukin BSF 2 antibody
- B cell differentiation factor antibody
- B cell stimulatory factor 2 antibody
see all
Images
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Lanes 1-2 : Anti-IL-6 antibody [EPR21710] (ab229381) at 1/1000 dilution
Lanes 3-4 : Anti-IL-6 antibody [EPR23819-11] (ab259341) at 1/1000 dilution
Lanes 1 & 3 : Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lanes 2 & 4 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 100ng/ml lipopolysaccharide (LPS) for 6 hours, then added 300 ng/ml BFA for the last 3 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
Exposure time: 180 secondsWestern blot analysis with ab229381 (left) and control product ab259341 (right).
Blocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-IL-6 antibody [EPR21710] (ab229381) at 1/1000 dilution
Lane 1 : Untreated RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus), whole cell lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with 100 ng/ml Lipopolysaccharides (LPS) for 6 hours, then added 300 ng/ml BFA for the last 3 hours, whole cell lysate, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 24 kDa
Observed band size: 24 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
We detect a non-specific band at approximately 60 KDa.
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IL6 was immunoprecipitated from 0.35 mg of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with 100 ng/ml Lipopolysaccharide (LPS) for 6 hours, then added 300 ng/ml BFA the last 3 hours, whole cell lysate with ab229381 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229381 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: RAW 264.7 treated with 100 ng/ml Lipopolysaccharide (LPS) for 6 hours, then added 300 ng/ml BFA the last 3 hours, whole cell lysate 10 µg (Input).
Lane 2: ab229381 IP in RAW 264.7 treated with 100 ng/ml Lipopolysaccharide (LPS) for 6 hours, then added 300 ng/ml BFA the last 3 hours, whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229381 in RAW 264.7 treated with 100 ng/ml Lipopolysaccharide (LPS) for 6 hours, then added 300 ng/ml BFA the last 3 hours, whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 2 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (20)
ab229381 has been referenced in 20 publications.
- Chen L et al. Naringenin is a Potential Immunomodulator for Inhibiting Liver Fibrosis by Inhibiting the cGAS-STING Pathway. J Clin Transl Hepatol 11:26-37 (2023). PubMed: 36406329
- Cui L et al. Circadian gene Rev-erbα influenced by sleep conduces to pregnancy by promoting endometrial decidualization via IL-6-PR-C/EBPβ axis. J Biomed Sci 29:101 (2022). PubMed: 36419076
- Prado NJ et al. Anti-inflammatory, antioxidant, antihypertensive, and antiarrhythmic effect of indole-3-carbinol, a phytochemical derived from cruciferous vegetables. Heliyon 8:e08989 (2022). PubMed: 35243102
- Olivera-Bravo S et al. Neuroprotective effects of violacein in a model of inherited amyotrophic lateral sclerosis. Sci Rep 12:4439 (2022). PubMed: 35292673
- Han X et al. TREM2 and CD163 Ameliorate Microglia-Mediated Inflammatory Environment in the Aging Brain. J Mol Neurosci 72:1075-1084 (2022). PubMed: 35306602