• Product name
  • Description
    Rabbit polyclonal to IL8
  • Host species
  • Specificity
    Please be aware that this product has low homology with the mouse and rat sequence of IL8 (Rat, 46%; Mouse 45%, UniProt blast) and we therefore cannot guarantee reactivity in these species.
  • Tested applications
    Suitable for: IHC-P, WB, IHC-Fr, IP, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Cynomolgus monkey, Rhesus monkey
  • Immunogen

    Recombinant full length protein (human)

  • Positive control



Our Abpromise guarantee covers the use of ab7747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 17208399
WB 1/10. Detects a band of approximately 6-8 kDa (predicted molecular weight: 11.1 kDa). IL-8 appears to exist as a dimer in solution.
IHC-Fr Use at an assay dependent concentration. PubMed: 25120588
IP 1/10.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 26270987


  • Function
    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • (Ala-IL-8)77 antibody
    • (Ser-IL-8)72 antibody
    • 9E3 antibody
    • Beta thromboglobulin like protein antibody
    • C-X-C motif chemokine 8 antibody
    • CEF-4 antibody
    • chemokine, CXC motif, ligand 8 antibody
    • CXCL8 antibody
    • Emoctakin antibody
    • GCP-1 antibody
    • GCP/IL-8 protein I antibody
    • GCP/IL-8 protein II antibody
    • GCP/IL-8 protein III antibody
    • GCP/IL-8 protein IV antibody
    • GCP/IL-8 protein V antibody
    • GCP/IL-8 protein VI antibody
    • GCP1 antibody
    • Granulocyte chemotactic protein 1 antibody
    • IL-8 antibody
    • IL-8(1-77) antibody
    • IL-8(9-77) antibody
    • IL8 antibody
    • IL8/NAP1 form I antibody
    • IL8/NAP1 form II antibody
    • IL8/NAP1 form III antibody
    • IL8/NAP1 form IV antibody
    • IL8/NAP1 form V antibody
    • IL8/NAP1 form VI antibody
    • IL8_HUMAN antibody
    • Inteleukin 8 antibody
    • LECT antibody
    • LUCT antibody
    • Lymphocyte-derived neutrophil-activating factor antibody
    • LYNAP antibody
    • MDNCF antibody
    • MDNCF-b antibody
    • MDNCF-c antibody
    • MONAP antibody
    • Monocyte-derived neutrophil chemotactic factor antibody
    • Monocyte-derived neutrophil-activating peptide antibody
    • NAF antibody
    • NAP-1 antibody
    • NAP1 antibody
    • Neutrophil activating peptide 1 antibody
    • Neutrophil activating protein 1 antibody
    • Neutrophil-activating factor antibody
    • Neutrophil-activating protein 1 antibody
    • Protein 3 10C antibody
    • Protein 3-10C antibody
    • SCYB8 antibody
    • Small inducible cytokine subfamily B member 8 antibody
    • T cell chemotactic factor antibody
    • T-cell chemotactic factor antibody
    • TSG1 antibody
    see all


  • ab7747 staining IL8 in SVGA astrocytes transfected with a plasmid encoding Vpr by ICC/IF (Immunocytochemistry/immunofluorescence). The cells were stained for nucleus (blue), IL8 (green) and GFAP (red). Cells were fixed with ice-cold methanol/acetone (1:1) for 20 minutes at 20°C, washed 3x with PBS and permeabilized with 0.1% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at RT. Samples were incubated with ab7747 overnight in a humidified chamber. The cells were washed 5x with 0.1% Triton X-100 in PBS and incubated for 1h in secondary antibodies conjugated with Alexa Fluor 488 (Anti-Rabbit, 1:1000) at room temperature in the dark. All the antibodies were diluted in PBS containing 1% BSA.


This product has been referenced in:
  • Saito K  et al. Xanthohumol inhibits angiogenesis by suppressing nuclear factor-?B activation in pancreatic cancer. Cancer Sci 109:132-140 (2018). Read more (PubMed: 29121426) »
  • de la Fuente C  et al. Phosphoproteomic analysis reveals Smad protein family activation following Rift Valley fever virus infection. PLoS One 13:e0191983 (2018). Read more (PubMed: 29408900) »

See all 25 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Cynomolgus Monkey Tissue sections (Macaque Retina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Macaque Retina
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 01 2016

Immunocytochemistry/ Immunofluorescence
Human Cell (Muller Cell Line MIO-M1)
Yes - 0.1% TritonX-100
Muller Cell Line MIO-M1
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5µg/mL · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 01 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (HNSCC Tissue)
Antigen retrieval step
Heat mediated
HNSCC Tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Dr. Biswanath Majumder

Verified customer

Submitted Jul 01 2015

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unseres Wissens nach wurde ab7747 bisher noch nicht in Durchflusszytometrie getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein sp...

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Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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Thank you for your reply.

In theory it is possible to perform IHC-P with a directly conjugated antibody. This will also mean that the amplification that is achieve by adding the secondary is lost.

So my advise would be to only do it...

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Thank you for your inquiry.

I am happy to confirm that the recommended protocol in IHC is the following:

Antigen retrieval: 5 min proteolytic enzyme (competitor), 20C

Antibody incubation: 120 min, 20C 1:500


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Thank you for your call today and for letting us know about the trouble with ab7747.

As we discussed, I'm sending a free of charge vial of ab18672 on the order *** which should arrive tomorrow.

Please keep me updated about the resul...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for your protocol, I have looked at it in details and I would like to suggest trying the following changes to see if you start having a cytoplasmic staining rather than a nuclear staining. -check you are not overfixing (or underfixing) the...

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