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Recombinant full length protein (human)
Our Abpromise guarantee covers the use of ab7747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 17208399|
|WB||1/10. Detects a band of approximately 6-8 kDa (predicted molecular weight: 11.1 kDa). IL-8 appears to exist as a dimer in solution.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 25120588|
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 26270987|
ab7747 staining IL8 in SVGA astrocytes transfected with a plasmid encoding Vpr by ICC/IF (Immunocytochemistry/immunofluorescence). The cells were stained for nucleus (blue), IL8 (green) and GFAP (red). Cells were fixed with ice-cold methanol/acetone (1:1) for 20 minutes at 20°C, washed 3x with PBS and permeabilized with 0.1% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at RT. Samples were incubated with ab7747 overnight in a humidified chamber. The cells were washed 5x with 0.1% Triton X-100 in PBS and incubated for 1h in secondary antibodies conjugated with Alexa Fluor 488 (Anti-Rabbit, 1:1000) at room temperature in the dark. All the antibodies were diluted in PBS containing 1% BSA.
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