Product nameAnti-ILF1 antibody
See all ILF1 primary antibodies
DescriptionRabbit polyclonal to ILF1
SpecificityThis antibody reacts with ILF1.
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- HepG2 cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab50946 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.25 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 69 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/12500.
FunctionRecognizes the core sequence 5'-TAAACA-3'. Binds to NFAT-like motifs (purine-rich) in the IL2 promoter. Also binds to HIV-1 long terminal repeat. May be involved in both positive and negative regulation of important viral and cellular promoter elements.
Tissue specificityExpressed in both lymphoid and non-lymphoid cells.
Sequence similaritiesContains 1 FHA domain.
Contains 1 fork-head DNA-binding domain.
DomainThe C-terminal part of the DNA-binding domain may contribute to DNA recognition specificity.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Cellular transcription factor ILF 1 antibody
- Cellular transcription factor ILF-1 antibody
- Cellular transcription factor ILF1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling ILF1 with ab50946 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the perinuclear/nuclear membrane. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - ILF1. Right - Merge.
Lane 2 : Anti-ILF1 antibody (ab50946) at 0.25 µg/ml
Lane 1 : MARKER
Lane 2 : HepG2 cell lysate at 10 µg
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 69 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
ab50946 has not yet been referenced specifically in any publications.