Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
ChIP Related Products
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab5298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration. Used in peptide ELISA against the immunogen. Detection limit >1/32,000|
|ChIP||Use at an assay dependent concentration. PubMed: 22083952This antibody is suitable for ChIP at some binding sites, but may not be sensitive enough to detect all FOXK2 binding sites.|
|ICC/IF||Use at an assay dependent dilution. PubMed: 20810654|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 75-80 kDa (predicted molecular weight: 73 kDa).Can be blocked with Human ILF1 peptide (ab23094).|
FunctionRecognizes the core sequence 5'-TAAACA-3'. Binds to NFAT-like motifs (purine-rich) in the IL2 promoter. Also binds to HIV-1 long terminal repeat. May be involved in both positive and negative regulation of important viral and cellular promoter elements.
Tissue specificityExpressed in both lymphoid and non-lymphoid cells.
Sequence similaritiesContains 1 FHA domain.
Contains 1 fork-head DNA-binding domain.
DomainThe C-terminal part of the DNA-binding domain may contribute to DNA recognition specificity.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Cellular transcription factor ILF 1 antibody
- Cellular transcription factor ILF-1 antibody
- Cellular transcription factor ILF1 antibody
Anti-ILF1 antibody - ChIP Grade (ab5298) at 0.3 µg/ml + Human thymus lysate in RIPA buffer at 35 µg
Predicted band size: 73 kDa
ab5298 at a 2
µg/ml concentration staining approximately 73 kDa ILF 1 in human heart lysate (30 µg total protein per lane) by Western blot (ECL). Primary incubation was 1 hour.
Immunofluorescence analysis of U2OS cells, staining ILF1 with ab5298. Cells were fixed with 4% paraformaldehyde and incubated with primary antibody at 1/100 dilution. A FITC-conjugated rabbit anti-goat IgG was used as the secondary antibody.
This product has been referenced in:
- Nestal de Moraes G et al. SUMOylation modulates FOXK2-mediated paclitaxel sensitivity in breast cancer cells. Oncogenesis 7:29 (2018). Read more (PubMed: 29540677) »
- Liu Y et al. FOXK2 transcription factor suppresses ERa-positive breast cancer cell growth through down-regulating the stability of ERa via mechanism involving BRCA1/BARD1. Sci Rep 5:8796 (2015). Read more (PubMed: 25740706) »