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Rabbit polyclonal to ILF2
Predicted to work with:
Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Orangutan, Xenopus tropicalis
Synthetic peptide, corresponding to a region within amino acids 340-390 of Human ILF2 (NP_004506.2)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 43 kDa.
Use at 2-10 µg/mg of lysate.
Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.
Contains 1 DZF domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Information by UniProt
ILF 2 antibody
Western blot - Anti-ILF2 antibody (ab113205)
All lanes : Anti-ILF2 antibody (ab113205) at 0.1 µg/ml Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg Lane 5 : NIH3T3 cell lysate at 50 µg Predicted band size: 43 kDa Exposure time: 10 seconds
Immunoprecipitation - Anti-ILF2 antibody (ab113205)
Detection of ILF2 by Western Blot of Immunprecipitate.
Lane 1: ab113205 at 1µg/ml staining ILF2 in HeLa whole cell lysate immunoprecipitated using ab113205 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
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