Overview

  • Product name
    Anti-ILF2 antibody [EPR10695(B)]
    See all ILF2 primary antibodies
  • Description
    Rabbit monoclonal [EPR10695(B)] to ILF2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, IPmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human ILF2 (UniProt Q12905).

  • Positive control
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
IHC-P 1/50 - 1/100.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function
      Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.
    • Sequence similarities
      Contains 1 DZF domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    • Information by UniProt
    • Database links
    • Alternative names
      • HGNC:6037 antibody
      • ILF 2 antibody
      • ilf2 antibody
      • ILF2_HUMAN antibody
      • Interleukin enhancer binding factor 2 antibody
      • Interleukin enhancer binding factor 2, 45kDa antibody
      • Interleukin enhancer-binding factor 2 antibody
      • MGC8391 antibody
      • NF 45 antibody
      • NF45 antibody
      • Nuclear factor of activated T cells 45 kDa antibody
      • Nuclear factor of activated T-cells 45 kDa antibody
      • PRO3063 antibody
      see all

    Images

    • All lanes : Anti-ILF2 antibody [EPR10695(B)] (ab154791) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 43 kDa

    • Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling ILF2 with ab154791 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling ILF2 with ab154791 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin embedded Human glioma tissue using ab154791 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab154791 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154791 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab154791 showing +ve staining.

    • Overlay histogram showing HeLa cells stained with ab154791 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab154791, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    References

    This product has been referenced in:
    • Bi Y  et al. MicroRNA-7 functions as a tumor-suppressor gene by regulating ILF2 in pancreatic carcinoma. Int J Mol Med 39:900-906 (2017). WB ; Human . Read more (PubMed: 28259961) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (4-12)
    Sample
    Human Cell lysate - whole cell (Lymphocytes)
    Specification
    Lymphocytes
    Treatment
    Various siRNA knockdown
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Oct 31 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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