Overview

  • Product name

  • Description

    Rabbit polyclonal to ILF2/NF45
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human ILF2/NF45 aa 200-300 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab104699)

  • Positive control

  • General notes

     This product was previously labelled as ILF2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab93838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • Function

    Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.
  • Sequence similarities

    Contains 1 DZF domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links

  • Alternative names

    • HGNC:6037 antibody
    • ILF 2 antibody
    • ilf2 antibody
    • ILF2_HUMAN antibody
    • Interleukin enhancer binding factor 2 antibody
    • Interleukin enhancer binding factor 2, 45kDa antibody
    • Interleukin enhancer-binding factor 2 antibody
    • MGC8391 antibody
    • NF 45 antibody
    • NF45 antibody
    • Nuclear factor of activated T cells 45 kDa antibody
    • Nuclear factor of activated T-cells 45 kDa antibody
    • PRO3063 antibody
    see all

Images

  • All lanes : Anti-ILF2/NF45 antibody (ab93838) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa
    Additional bands at: 100 kDa, 22 kDa, 65 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes

References

ab93838 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab93838.
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