Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab154169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.
    • Sequence similarities

      Contains 1 DZF domain.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    • Information by UniProt
    • Database links

    • Alternative names

      • HGNC:6037 antibody
      • ILF 2 antibody
      • ilf2 antibody
      • ILF2_HUMAN antibody
      • Interleukin enhancer binding factor 2 antibody
      • Interleukin enhancer binding factor 2, 45kDa antibody
      • Interleukin enhancer-binding factor 2 antibody
      • MGC8391 antibody
      • NF 45 antibody
      • NF45 antibody
      • Nuclear factor of activated T cells 45 kDa antibody
      • Nuclear factor of activated T-cells 45 kDa antibody
      • PRO3063 antibody
      see all

    Images

    • All lanes : Anti-ILF2/NF45 antibody [EPR10694(B)] (ab154169) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : HepG2 cell lysates
      Lane 3 : Jurkat cell lysates
      Lane 4 : K562 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 43 kDa

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling ILF2/NF45 with ab154169 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ILF2/NF45 with ab154169 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded Human normal stomach tissue using ab154169 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded Human normal tetis tissue using ab154169 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab154169 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab154169 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Du H  et al. ILF2 Directly Binds and Stabilizes CREB to Stimulate Malignant Phenotypes of Liver Cancer Cells. Anal Cell Pathol (Amst) 2019:1575031 (2019). Read more (PubMed: 30881868) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Primary macrophage)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    85 µg
    Specification
    Primary macrophage
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

    Dr. David Gregory

    Verified customer

    Submitted Mar 20 2015

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