Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab131004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 95 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N-methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 DZF domain.
  • Post-translational
    modifications

    Phosphorylated by RNA-dependent protein kinase (EIF2AK2).
    Methylated by protein arginine N-methyltransferase 1.
    Arg-609 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization

    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links

  • Alternative names

    • CBTF antibody
    • Double stranded RNA binding protein 76 antibody
    • Double-stranded RNA-binding protein 76 antibody
    • DRBF antibody
    • DRBP76 antibody
    • ILF3 antibody
    • ILF3_HUMAN antibody
    • Interleukin enhancer binding factor 3 antibody
    • Interleukin enhancer-binding factor 3 antibody
    • M phase phosphoprotein 4 antibody
    • M-phase phosphoprotein 4 antibody
    • MPHOSPH4 antibody
    • MPP4 antibody
    • NF AT 90 antibody
    • NF-AT-90 antibody
    • NF110 antibody
    • NF90 antibody
    • NFAR antibody
    • Nuclear factor associated with dsRNA antibody
    • Nuclear factor of activated T cells 90 kDa antibody
    • Nuclear factor of activated T-cells 90 kDa antibody
    • TCP80 antibody
    • Translational control protein 80 antibody
    see all

Images

  • All lanes : Anti-ILF3/NF90 antibody (ab131004) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 95 kDa


    Exposure time: 10 seconds
  • Detection of ILF3/NF90 by Western Blot of Immunprecipitate.

    ab131004 at 1µg/ml staining ILF3/NF90 in HeLa whole cell lysate immunoprecipitated using ab131004 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

    Detection: Chemiluminescence with exposure time of 10 seconds.

References

This product has been referenced in:

  • Jiang Z  et al. LincIN, a novel NF90-binding long non-coding RNA, is overexpressed in advanced breast tumors and involved in metastasis. Breast Cancer Res 19:62 (2017). Read more (PubMed: 28558830) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Prostate cancer cells)
Total protein in input
500 µg
Immuno-precipitation step
Protein A
Specification
Prostate cancer cells

Abcam user community

Verified customer

Submitted Apr 30 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up