Overview

  • Product name

  • Description

    Rabbit polyclonal to ILF3/NF90
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human ILF3/NF90 aa 325-375. The exact sequence is proprietary. (NP_036350.2).
    Database link: Q12906

  • Positive control

    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysate. IHC-P: Human ovarian cancer tissue; Mouse teratoma tissue.
  • General notes

     This product was previously labelled as ILF3

     

Properties

Applications

Our Abpromise guarantee covers the use of ab225619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 95 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N-methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Contains 2 DRBM (double-stranded RNA-binding) domains.
      Contains 1 DZF domain.
    • Post-translational
      modifications

      Phosphorylated by RNA-dependent protein kinase (EIF2AK2).
      Methylated by protein arginine N-methyltransferase 1.
      Arg-609 is dimethylated, probably to asymmetric dimethylarginine.
    • Cellular localization

      Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    • Information by UniProt
    • Database links

    • Alternative names

      • CBTF antibody
      • Double stranded RNA binding protein 76 antibody
      • Double-stranded RNA-binding protein 76 antibody
      • DRBF antibody
      • DRBP76 antibody
      • ILF3 antibody
      • ILF3_HUMAN antibody
      • Interleukin enhancer binding factor 3 antibody
      • Interleukin enhancer-binding factor 3 antibody
      • M phase phosphoprotein 4 antibody
      • M-phase phosphoprotein 4 antibody
      • MPHOSPH4 antibody
      • MPP4 antibody
      • NF AT 90 antibody
      • NF-AT-90 antibody
      • NF110 antibody
      • NF90 antibody
      • NFAR antibody
      • Nuclear factor associated with dsRNA antibody
      • Nuclear factor of activated T cells 90 kDa antibody
      • Nuclear factor of activated T-cells 90 kDa antibody
      • TCP80 antibody
      • Translational control protein 80 antibody
      see all

    Images

    • All lanes : Anti-ILF3/NF90 antibody (ab225619) at 1/2000 dilution

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
      Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 95 kDa


      Exposure time: 3 minutes
    • Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for ILF3/NF90 with ab225619 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.

    • Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for ILF3/NF90 with ab225619 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.

    References

    This product has been referenced in:

    • Du H  et al. ILF2 Directly Binds and Stabilizes CREB to Stimulate Malignant Phenotypes of Liver Cancer Cells. Anal Cell Pathol (Amst) 2019:1575031 (2019). Read more (PubMed: 30881868) »
    See 1 Publication for this product

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