• Product name

  • Description

    Rabbit polyclonal to ILF3/NF90
  • Host species

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human ILF3/NF90 aa 325-375. The exact sequence is proprietary. (NP_036350.2).
    Database link: Q12906

  • Positive control

    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysate. IHC-P: Human ovarian cancer tissue; Mouse teratoma tissue.
  • General notes

     This product was previously labelled as ILF3




Our Abpromise guarantee covers the use of ab225619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 95 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N-methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.
    • Tissue specificity

    • Sequence similarities

      Contains 2 DRBM (double-stranded RNA-binding) domains.
      Contains 1 DZF domain.
    • Post-translational

      Phosphorylated by RNA-dependent protein kinase (EIF2AK2).
      Methylated by protein arginine N-methyltransferase 1.
      Arg-609 is dimethylated, probably to asymmetric dimethylarginine.
    • Cellular localization

      Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    • Information by UniProt
    • Database links

    • Alternative names

      • CBTF antibody
      • Double stranded RNA binding protein 76 antibody
      • Double-stranded RNA-binding protein 76 antibody
      • DRBF antibody
      • DRBP76 antibody
      • ILF3 antibody
      • ILF3_HUMAN antibody
      • Interleukin enhancer binding factor 3 antibody
      • Interleukin enhancer-binding factor 3 antibody
      • M phase phosphoprotein 4 antibody
      • M-phase phosphoprotein 4 antibody
      • MPHOSPH4 antibody
      • MPP4 antibody
      • NF AT 90 antibody
      • NF-AT-90 antibody
      • NF110 antibody
      • NF90 antibody
      • NFAR antibody
      • Nuclear factor associated with dsRNA antibody
      • Nuclear factor of activated T cells 90 kDa antibody
      • Nuclear factor of activated T-cells 90 kDa antibody
      • TCP80 antibody
      • Translational control protein 80 antibody
      see all


    • All lanes : Anti-ILF3/NF90 antibody (ab225619) at 1/2000 dilution

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
      Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 95 kDa

      Exposure time: 3 minutes
    • Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for ILF3/NF90 with ab225619 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.

    • Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for ILF3/NF90 with ab225619 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.


    This product has been referenced in:

    • Du H  et al. ILF2 Directly Binds and Stabilizes CREB to Stimulate Malignant Phenotypes of Liver Cancer Cells. Anal Cell Pathol (Amst) 2019:1575031 (2019). Read more (PubMed: 30881868) »
    See 1 Publication for this product

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