Overview

  • Product name

    Anti-ILF3/NF90 antibody [EPR3627]
    See all ILF3/NF90 primary antibodies
  • Description

    Rabbit monoclonal [EPR3627] to ILF3/NF90
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ILF3/NF90 (C terminal). The exact sequence is proprietary.
    Database link: Q12906

  • Positive control

    • HeLa, K562 and A431 cell lysates; Human kidney tissue; HeLa and A431 cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as ILF3

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/2000 - 1/5000. Predicted molecular weight: 95 kDa.
IP 1/50.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N-methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Contains 2 DRBM (double-stranded RNA-binding) domains.
      Contains 1 DZF domain.
    • Post-translational
      modifications

      Phosphorylated by RNA-dependent protein kinase (EIF2AK2).
      Methylated by protein arginine N-methyltransferase 1.
      Arg-609 is dimethylated, probably to asymmetric dimethylarginine.
    • Cellular localization

      Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    • Information by UniProt
    • Database links

    • Alternative names

      • CBTF antibody
      • Double stranded RNA binding protein 76 antibody
      • Double-stranded RNA-binding protein 76 antibody
      • DRBF antibody
      • DRBP76 antibody
      • ILF3 antibody
      • ILF3_HUMAN antibody
      • Interleukin enhancer binding factor 3 antibody
      • Interleukin enhancer-binding factor 3 antibody
      • M phase phosphoprotein 4 antibody
      • M-phase phosphoprotein 4 antibody
      • MPHOSPH4 antibody
      • MPP4 antibody
      • NF AT 90 antibody
      • NF-AT-90 antibody
      • NF110 antibody
      • NF90 antibody
      • NFAR antibody
      • Nuclear factor associated with dsRNA antibody
      • Nuclear factor of activated T cells 90 kDa antibody
      • Nuclear factor of activated T-cells 90 kDa antibody
      • TCP80 antibody
      • Translational control protein 80 antibody
      see all

    Images

    • All lanes : Anti-ILF3/NF90 antibody [EPR3627] (ab133354) at 1/2000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 95 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling ILF3/NF90 with ab133354 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunofluorescent analysis of HeLa cells labelling ILF3/NF90 with ab133354 1/100 dilution.

    • Immunofluorescent analysis of A431 cells labelling ILF3/NF90 with ab133354 1/100 dilution.

    • Immunohistochemical analysis of paraffin embedded normal Human lung tissue using ab133354 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab133354 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab133354 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded Human Endometrial carcinoma tissue using ab133354 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded Human Clear cell carcinoma of kidney tissue using ab133354 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded normal Human skin tissue using ab133354 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Sokol ES  et al. SMARCE1 is required for the invasive progression of in situ cancers. Proc Natl Acad Sci U S A 114:4153-4158 (2017). WB ; Human . Read more (PubMed: 28377514) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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