Anti-ILKAP antibody (ab229056)
Key features and details
- Rabbit polyclonal to ILKAP
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ILKAP antibody
See all ILKAP primary antibodies -
Description
Rabbit polyclonal to ILKAP -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment within Human ILKAP (internal sequence). The exact sequence is proprietary.
Database link: Q9H0C8 -
Positive control
- WB: MCF7 whole cell lysate. ICC/IF: MCF7 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab229056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/500 - 1/3000. Predicted molecular weight: 43 kDa. | |
ICC/IF | 1/100 - 1/1000. |
Target
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Function
Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation. -
Tissue specificity
Widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues. -
Sequence similarities
Belongs to the PP2C family.
Contains 1 PP2C-like domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 514223 Cow
- Entrez Gene: 80895 Human
- Entrez Gene: 67444 Mouse
- Entrez Gene: 64538 Rat
- SwissProt: Q0IIF0 Cow
- SwissProt: Q9H0C8 Human
- SwissProt: Q8R0F6 Mouse
- SwissProt: Q9Z1Z6 Rat
see all -
Alternative names
- DKFZp434J2031 antibody
- FLJ10181 antibody
- ILKAP antibody
see all
Images
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Anti-ILKAP antibody (ab229056) at 1/1000 dilution + MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 43 kDa10% SDS-PAGE
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Methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for ILKAP (green) using ab229056 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab229056 has not yet been referenced specifically in any publications.