Anti-ILKAP antibody (ab229056)

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Overview

  • Product name

    Anti-ILKAP antibody
    See all ILKAP primary antibodies

  • Description

    Rabbit polyclonal to ILKAP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow

  • Immunogen

    Recombinant fragment within Human ILKAP (internal sequence). The exact sequence is proprietary.
    Database link: Q9H0C8

  • Positive control

    • WB: MCF7 whole cell lysate. ICC/IF: MCF7 cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 43 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation.

  • Tissue specificity

    Widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues.

  • Sequence similarities

    Belongs to the PP2C family.
    Contains 1 PP2C-like domain.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • DKFZp434J2031 antibody
    • FLJ10181 antibody
    • ILKAP antibody
    • ILKAP_HUMAN antibody
    • ILKAP2 antibody
    • ILKAP3 antibody
    • Integrin linked kinase associated phosphatase antibody
    • Integrin linked kinase associated serine/threonine phosphatase 2C antibody
    • Integrin linked kinase associated serine/threonine phosphatase antibody
    • Integrin-linked kinase-associated serine/threonine phosphatase 2C antibody
    • MGC4846 antibody
    • PP2C DELTA antibody
    • Protein phosphatase 2c delta isozyme antibody
    see all

Images

  • Western blot - Anti-ILKAP antibody (ab229056)
    Western blot - Anti-ILKAP antibody (ab229056)
    Anti-ILKAP antibody (ab229056) at 1/1000 dilution + MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 43 kDa



    10% SDS-PAGE

  • Immunocytochemistry/ Immunofluorescence - Anti-ILKAP antibody (ab229056)
    Immunocytochemistry/ Immunofluorescence - Anti-ILKAP antibody (ab229056)

    Methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for ILKAP (green) using ab229056 at 1/200 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab229056 has not yet been referenced specifically in any publications.

Publishing research using ab229056? Please let us know so that we can cite the reference in this datasheet.

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