Anti-ILKAP antibody (ab229056)

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Western blot - Anti-ILKAP antibody (ab229056)
  • Immunocytochemistry/ Immunofluorescence - Anti-ILKAP antibody (ab229056)

Key features and details

  • Rabbit polyclonal to ILKAP
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-ILKAP antibody
    See all ILKAP primary antibodies

  • Description

    Rabbit polyclonal to ILKAP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow

  • Immunogen

    Recombinant fragment within Human ILKAP (internal sequence). The exact sequence is proprietary.
    Database link: Q9H0C8

  • Positive control

    • WB: MCF7 whole cell lysate. ICC/IF: MCF7 cells.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 43 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation.

  • Tissue specificity

    Widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues.

  • Sequence similarities

    Belongs to the PP2C family.
    Contains 1 PP2C-like domain.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • DKFZp434J2031 antibody
    • FLJ10181 antibody
    • ILKAP antibody
    • ILKAP_HUMAN antibody
    • ILKAP2 antibody
    • ILKAP3 antibody
    • Integrin linked kinase associated phosphatase antibody
    • Integrin linked kinase associated serine/threonine phosphatase 2C antibody
    • Integrin linked kinase associated serine/threonine phosphatase antibody
    • Integrin-linked kinase-associated serine/threonine phosphatase 2C antibody
    • MGC4846 antibody
    • PP2C DELTA antibody
    • Protein phosphatase 2c delta isozyme antibody
    see all

Images

  • Western blot - Anti-ILKAP antibody (ab229056)
    Western blot - Anti-ILKAP antibody (ab229056)
    Anti-ILKAP antibody (ab229056) at 1/1000 dilution + MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 43 kDa



    10% SDS-PAGE

  • Immunocytochemistry/ Immunofluorescence - Anti-ILKAP antibody (ab229056)
    Immunocytochemistry/ Immunofluorescence - Anti-ILKAP antibody (ab229056)

    Methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for ILKAP (green) using ab229056 at 1/200 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab229056? Please let us know so that we can cite the reference in this datasheet.

ab229056 has not yet been referenced specifically in any publications.

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