Overview

  • Product name

    Anti-ILKAP antibody [EPR16145]
    See all ILKAP primary antibodies
  • Description

    Rabbit monoclonal [EPR16145] to ILKAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ILKAP aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9H0C8

  • Positive control

    • HeLa, 293, Mouse brain and Rat brain lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16145
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab196013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IP 1/30.

Target

  • Function

    Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation.
  • Tissue specificity

    Widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues.
  • Sequence similarities

    Belongs to the PP2C family.
    Contains 1 PP2C-like domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp434J2031 antibody
    • FLJ10181 antibody
    • ILKAP antibody
    • ILKAP_HUMAN antibody
    • ILKAP2 antibody
    • ILKAP3 antibody
    • Integrin linked kinase associated phosphatase antibody
    • Integrin linked kinase associated serine/threonine phosphatase 2C antibody
    • Integrin linked kinase associated serine/threonine phosphatase antibody
    • Integrin-linked kinase-associated serine/threonine phosphatase 2C antibody
    • MGC4846 antibody
    • PP2C DELTA antibody
    • Protein phosphatase 2c delta isozyme antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: ILKAP knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HEK293 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab196013 observed at 43 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab196013 was shown to recognize ILKAP when ILKAP knockout samples were used, along with additional cross-reactive bands. Wild-type and ILKAP knockout samples were subjected to SDS-PAGE. Ab196013 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Anti-ILKAP antibody [EPR16145] (ab196013) at 1/20000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa

  • Anti-ILKAP antibody [EPR16145] (ab196013) at 1/20000 dilution + 293 (Human epithelial cells from embryonic kidney) cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa

  • Anti-ILKAP antibody [EPR16145] (ab196013) at 1/5000 dilution + Mouse brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa

  • Anti-ILKAP antibody [EPR16145] (ab196013) at 1/1000 dilution + Rat brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa

  • Immunoprecipitation analysis of 293 (Human epithelial cells from embryonic kidney) whole cell extract labeling ILKAP using ab196013 at 1/30 dilution (Lane 2).
    Lane 3: IP using Rabbit monoclonal IgG (ab172730) instead of ab196013 in 293 (Human epithelial cells from embryonic kidney) whole cell extract.
    Lane 1: Input: 10 μg 293 (Human epithelial cells from embryonic kidney) whole cell extract.
    Subsequent WB detection was performed using ab196013 at 1/1000 dilution.
    An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

References

This product has been referenced in:

  • Liu L  et al. HMGA1 participates in MHCC97H cell proliferation and invasion through the ILK/Akt/GSK3ß signaling pathway. Mol Med Rep 16:9287-9294 (2017). Read more (PubMed: 29152644) »
See 1 Publication for this product

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