Anti-ILP2 antibody (ab9664)
Key features and details
- Rabbit polyclonal to ILP2
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ILP2 antibody -
Description
Rabbit polyclonal to ILP2 -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC HumanWB MouseHuman -
Immunogen
Synthetic peptide:
TGYEARLITFGT
, corresponding to amino acids 2-13 of Human ILP2. (Peptide available as ab9665.) -
General notes
Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family including IAP, XIAP/ILP-1/MIHA, and Livin/KIAP that bind to and inhibits specific proteases. A novel member in the IAP protein family was recently identified and designated ILP-2 for IAP-like protein-2 (1). ILP-2 has high homology to ILP-1, but is encoded by a distinct gene that is solely expressed in testis of tested normal human tissues (1). ILP-2, unlike ILP-1, has no inhibitory effect on Fas and TNF induced apoptosis, but potently inhibits apoptosis induced by overexpression of Bax or by coexpression of caspase-9 with Apaf-1. ILP-2 interacts with the processed caspase-9. These results suggest that ILP-2 is a novel IAP family member with restricted specificity for caspase-9.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family including IAP, XIAP/ILP-1/MIHA, and Livin/KIAP that bind to and inhibits specific proteases. A novel member in the IAP protein family was recently identified and designated ILP-2 for IAP-like protein-2 (1). ILP-2 has high homology to ILP-1, but is encoded by a distinct gene that is solely expressed in testis of tested normal human tissues (1). ILP-2, unlike ILP-1, has no inhibitory effect on Fas and TNF induced apoptosis, but potently inhibits apoptosis induced by overexpression of Bax or by coexpression of caspase-9 with Apaf-1. ILP-2 interacts with the processed caspase-9. These results suggest that ILP-2 is a novel IAP family member with restricted specificity for caspase-9. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9664 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
ICC |
Human
|
WB |
Mouse
Human
|
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 33 kDa.
|
|
ICC |
Use a concentration of 10 µg/ml.
|
Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 33 kDa. |
ICC
Use a concentration of 10 µg/ml. |
Target
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Function
Protects against apoptosis mediated by BAX. -
Tissue specificity
Testis specific in normal tissues. -
Sequence similarities
Belongs to the IAP family.
Contains 1 BIR repeat.
Contains 1 RING-type zinc finger. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 112401 Human
- SwissProt: Q96P09 Human
- Unigene: 348263 Human
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Alternative names
- Baculoviral IAP repeat containing 8 antibody
- Baculoviral IAP repeat containing protein 8 antibody
- Baculoviral IAP repeat-containing protein 8 antibody
see all
Images
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Western blot analysis of ILP-2 expression in human HepG2 (lane 1) and MOLT4 (lane 2) cell lysates and mouse spleen (lane 3) tissue lysates with anti-ILP-2 at 1
Western blot analysis of ILP-2 expression in human HepG2 (lane 1) and MOLT4 (lane 2) cell lysates and mouse spleen (lane 3) tissue lysates with anti-ILP-2 at 1 µg/ml.µ g/ml. -
ab9664 at 10µg/ml staining ILP2 in HepG2 cells by Immunocytochemistry.
Datasheets and documents
References (2)
ab9664 has been referenced in 2 publications.
- Kong D et al. Protective effects of Asiatic acid against pelvic inflammatory disease in rats. Exp Ther Med 17:4687-4692 (2019). PubMed: 31086602
- Zhu L et al. Inhibitor of apoptosis protein-like protein-2: A novel growth accelerator for breast cancer cells. Oncol Rep 40:2047-2055 (2018). PubMed: 30106449