Key features and details
- Rabbit polyclonal to ILT-3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ILT-3 antibody
See all ILT-3 primary antibodies
DescriptionRabbit polyclonal to ILT-3
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human ILT-3 aa 22-259. Extracellular domain.
QAGPLPKPTLWAEPGSVISWGNSVTIWCQGTLEAREYRLDKEESPAPWDR QNPLEPKNKARFSIPSMTEDYAGRYRCYYRSPVGWSQPSDPLELVMTGAY SKPTLSALPSPLVTSGKSVTLLCQSRSPMDTFLLIKERAAHPLLHLRSEH GAQQHQAEFPMSPVTSVHGGTYRCFSSHGFSHYLLSHPSDPLELIVSGSL EDPRPSPTRSVSTAAGPEDQPLMPTGSVPHSGLRRHWE
Database link: Q8NHJ6
- WB: MCF7 and A549 whole cell lysates. IHC-P: Human lung tissue.
Previously labelled as LILRB4.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).|
FunctionReceptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance, e.g. towards transplants. Interferes with TNFRSF5-signaling and NF-kappa-B up-regulation. Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
Tissue specificityDetected in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells.
Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
DomainContains 3 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Cellular localizationCell membrane. Ligand binding leads to internalization and translocation to an antigen-processing compartment.
- Information by UniProt
- CD85 antigen-like family member K antibody
- CD85k antibody
- HM18 antibody
All lanes : Anti-ILT-3 antibody (ab229747) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Paraffin-embedded human lung tissue stained for ILT-3 using ab229747 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229747 has not yet been referenced specifically in any publications.