Recombinant Anti-ILT-3 antibody [EPR22030] (ab224716)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22030] to ILT-3
- Suitable for: Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-ILT-3 antibody [EPR22030]
See all ILT-3 primary antibodies -
Description
Rabbit monoclonal [EPR22030] to ILT-3 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human ILT-3 aa 100-350. The exact sequence is proprietary.
Database link: Q8NHJ6 -
Positive control
- Flow Cyt: Human peripheral blood mononuclear cells (PBMCs).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22030 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab224716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/600. |
Target
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Function
Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance, e.g. towards transplants. Interferes with TNFRSF5-signaling and NF-kappa-B up-regulation. Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. -
Tissue specificity
Detected in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
Contains 3 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Cellular localization
Cell membrane. Ligand binding leads to internalization and translocation to an antigen-processing compartment. - Information by UniProt
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Database links
- Entrez Gene: 11006 Human
- Omim: 604821 Human
- SwissProt: Q8NHJ6 Human
- Unigene: 67846 Human
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Alternative names
- CD85 antigen-like family member K antibody
- CD85k antibody
- HM18 antibody
see all
Images
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![Flow Cytometry - Anti-ILT-3 antibody [EPR22030] (ab224716)](https://www.abcam.com/ps/products/224/ab224716/Images/ab224716-334594-anti-ilt-3-antibody-epr22030-flow-cytometry.jpg)
Flow Cytometry - Anti-ILT-3 antibody [EPR22030] (ab224716)Flow cytometric analysis of human peripheral blood mononuclear cells (PBMCs) labeling ILT-3 with ab224716 at 1/600 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Dylight® 488) (ab98462) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with Alexa Fluor® 647-conjugated CD14 (a monocyte marker) and rabbit IgG (left, ab172370) or anti-ILT-3 RabMAb (right, ab224716). Data shown was gated on viable cells.
The ILT-3 expression pattern has been described in literature (PMID:17266150; PMID:9151699).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab224716 has not yet been referenced specifically in any publications.
