Recombinant Anti-ILT-3 antibody [EPR22030] - BSA and Azide free (ab244552)


  • Product name

    Anti-ILT-3 antibody [EPR22030] - BSA and Azide free
    See all ILT-3 primary antibodies
  • Description

    Rabbit monoclonal [EPR22030] to ILT-3 - BSA and Azide free
  • Host species

  • Tested applications

    Suitable for: Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human ILT-3 aa 100-350. The exact sequence is proprietary.
    Database link: Q8NHJ6

  • Positive control

    • Flow Cyt: Human peripheral blood mononuclear cells (PBMCs).
  • General notes

    Ab244552 is the carrier-free version of ab224716. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.


    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab244552 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab244552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or WB.
  • Target

    • Function

      Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance, e.g. towards transplants. Interferes with TNFRSF5-signaling and NF-kappa-B up-regulation. Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
    • Tissue specificity

      Detected in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells.
    • Sequence similarities

      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • Domain

      Contains 3 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • Cellular localization

      Cell membrane. Ligand binding leads to internalization and translocation to an antigen-processing compartment.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD85 antigen-like family member K antibody
      • CD85k antibody
      • HM18 antibody
      • ILT-3 antibody
      • ILT3 antibody
      • Immunoglobulin-like transcript 3 antibody
      • Leukocyte immunoglobulin like receptor subfamily B (with TM and ITIM domains) member 4 antibody
      • Leukocyte immunoglobulin-like receptor 5 antibody
      • Leukocyte immunoglobulin-like receptor subfamily B member 4 antibody
      • LILRB4 antibody
      • LILRB5 antibody
      • LIR-5 antibody
      • LIR5 antibody
      • LIRB4_HUMAN antibody
      • Monocyte inhibitory receptor HM18 antibody
      see all


    • Flow cytometric analysis of human peripheral blood mononuclear cells (PBMCs) labeling ILT-3 with ab224716 at 1/600 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Dylight® 488) (ab98462) at 1/2000 dilution was used as the secondary antibody.

      Cells were stained with Alexa Fluor® 647-conjugated CD14 (a monocyte marker) and rabbit IgG (left, ab172370) or anti-ILT-3 RabMAb (right, ab224716). Data shown was gated on viable cells.

      The ILT-3 expression pattern has been described in literature (PMID:17266150; PMID:9151699).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224716).


    ab244552 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab244552.
    Please use the links above to contact us or submit feedback about this product.

    For licensing inquiries, please contact

    Sign up