Recombinant
RabMAb

Recombinant Anti-ILT-3 antibody [EPR22030] - BSA and Azide free (ab244552)

Overview

  • Product name

    Anti-ILT-3 antibody [EPR22030] - BSA and Azide free
    See all ILT-3 primary antibodies
  • Description

    Rabbit monoclonal [EPR22030] to ILT-3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human ILT-3 aa 100-350. The exact sequence is proprietary.
    Database link: Q8NHJ6

  • Positive control

    • Flow Cyt: Human peripheral blood mononuclear cells (PBMCs).
  • General notes

    Ab244552 is the carrier-free version of ab224716. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab244552 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab244552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or WB.
  • Target

    • Function

      Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance, e.g. towards transplants. Interferes with TNFRSF5-signaling and NF-kappa-B up-regulation. Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
    • Tissue specificity

      Detected in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells.
    • Sequence similarities

      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • Domain

      Contains 3 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • Cellular localization

      Cell membrane. Ligand binding leads to internalization and translocation to an antigen-processing compartment.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD85 antigen-like family member K antibody
      • CD85k antibody
      • HM18 antibody
      • ILT-3 antibody
      • ILT3 antibody
      • Immunoglobulin-like transcript 3 antibody
      • Leukocyte immunoglobulin like receptor subfamily B (with TM and ITIM domains) member 4 antibody
      • Leukocyte immunoglobulin-like receptor 5 antibody
      • Leukocyte immunoglobulin-like receptor subfamily B member 4 antibody
      • LILRB4 antibody
      • LILRB5 antibody
      • LIR-5 antibody
      • LIR5 antibody
      • LIRB4_HUMAN antibody
      • Monocyte inhibitory receptor HM18 antibody
      see all

    Images

    • Flow cytometric analysis of human peripheral blood mononuclear cells (PBMCs) labeling ILT-3 with ab224716 at 1/600 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Dylight® 488) (ab98462) at 1/2000 dilution was used as the secondary antibody.

      Cells were stained with Alexa Fluor® 647-conjugated CD14 (a monocyte marker) and rabbit IgG (left, ab172370) or anti-ILT-3 RabMAb (right, ab224716). Data shown was gated on viable cells.

      The ILT-3 expression pattern has been described in literature (PMID:17266150; PMID:9151699).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224716).

    References

    ab244552 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab244552.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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