Recombinant
RabMAb

Recombinant Anti-ILT-4 antibody [EPR22081] - BSA and Azide free (ab243107)

Overview

  • Product name

    Anti-ILT-4 antibody [EPR22081] - BSA and Azide free
    See all ILT-4 primary antibodies
  • Description

    Rabbit monoclonal [EPR22081] to ILT-4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human ILT-4 aa 1-250. The exact sequence is proprietary.
    Database link: Q8N423

  • Positive control

    • ICC/IF: Human PBMC. Flow Cyt: Human PBMC.
  • General notes

    Ab243107 is the carrier-free version of ab224701. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab243107 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22081
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab243107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P or WB.
  • Target

    • Function

      Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance. Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
    • Tissue specificity

      Expressed on monocytes and B-cells, and at lower levels on dendritic cells. Detected at low levels in natural killer (NK) cells.
    • Sequence similarities

      Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
    • Domain

      Contains 3 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • Post-translational
      modifications

      Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD85 antigen-like family member D antibody
      • CD85d antibody
      • CD85d antigen antibody
      • ILT 4 antibody
      • ILT-4 antibody
      • ILT4 antibody
      • Immunoglobulin like transcript 4 antibody
      • Immunoglobulin-like transcript 4 antibody
      • Leukocyte immunoglobulin like receptor 2 antibody
      • Leukocyte immunoglobulin like receptor subfamily B member 2 antibody
      • Leukocyte immunoglobulin-like receptor 2 antibody
      • Leukocyte immunoglobulin-like receptor subfamily B member 2 antibody
      • Leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2 antibody
      • LILRA6 antibody
      • LILRB2 antibody
      • LIR 2 antibody
      • LIR-2 antibody
      • LIR2 antibody
      • LIRb antibody
      • LIRB2_HUMAN antibody
      • MIR 10 antibody
      • MIR-10 antibody
      • MIR10 antibody
      • Monocyte/macrophage immunoglobulin like receptor 10 antibody
      • Monocyte/macrophage immunoglobulin-like receptor 10 antibody
      see all

    Images

    • Flow cytometric analysis of human primary peripheral blood mononuclear cell (PBMC) labeling ILT-4 with ab224701 at 1/500 (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat anti rabbit IgG (Dylight ® 488, ab98462) at 1/2000 dilution was used as the secondary antibody.

      Cells were stained with Alexa Fluor® 647-conjugated CD14 and rabbit IgG (Left) or ILT-4 (Right).

      Data shown were gated on viable cells.

      The CD14 co-staining result observed is consistent with what has been described in the literature (PMID:10879687).

      ILT-4 is expressed in monocytes and CD14 is an established marker for monocytes and macrophages (PMID: 23382732).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224701).

    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Human PBMC (human primary peripheral blood mononuclear cell) cells labeling ILT-4 with ab224701 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing co-staining with CD14 and cytoplasmic staining in PBMC cells. The nuclear counterstain is DAPI (blue). Counterstained with an Alexa Fluor® 647 anti-human CD14 antibody at a 1/100 dilution (red).

      The negative control is the secondary antibody only.

      ILT-4 is expressed in monocytes (PMID:10879687) and CD14 is an established marker for monocytes and macrophages (PMID: 23382732).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224701).

    References

    ab243107 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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