Recombinant Anti-ILT-4 antibody [EPR22081] - Low endotoxin, Azide free (ab246796)
![Flow Cytometry - Anti-ILT-4 antibody [EPR22081] - Low endotoxin, Azide free (ab246796)](https://www.abcam.com/ps/products/246/ab246796/Images/ab246796-2-ab2247012224701FC1.jpg "Flow Cytometry - Anti-ILT-4 antibody [EPR22081] - Low endotoxin, Azide free (ab246796)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22081] to ILT-4 - Low endotoxin, Azide free
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-ILT-4 antibody [EPR22081] - Low endotoxin, Azide free
See all ILT-4 primary antibodies -
Description
Rabbit monoclonal [EPR22081] to ILT-4 - Low endotoxin, Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human ILT-4 aa 1-250. The exact sequence is proprietary.
Database link: Q8N423 -
Positive control
- ICC/IF: Human PBMC. Flow Cyt: Human PBMC.
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General notes
ab246796 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Endotoxin level is less than 1 EU/ml as determined by the TAL test -
Clonality
Monoclonal -
Clone number
EPR22081 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab246796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. |
Target
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Function
Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance. Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. -
Tissue specificity
Expressed on monocytes and B-cells, and at lower levels on dendritic cells. Detected at low levels in natural killer (NK) cells. -
Sequence similarities
Contains 4 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
Contains 3 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Post-translational
modificationsPhosphorylated on tyrosine residues. Dephosphorylated by PTPN6. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10288 Human
- Omim: 604815 Human
- SwissProt: Q8N423 Human
- Unigene: 655652 Human
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Alternative names
- CD85 antigen-like family member D antibody
- CD85d antibody
- CD85d antigen antibody
see all
Images
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Flow Cytometry - Anti-ILT-4 antibody [EPR22081] - Low endotoxin, Azide free (ab246796)
Flow cytometric analysis of human primary peripheral blood mononuclear cell (PBMC) labeling ILT-4 with ab224701 at 1/500 (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat anti rabbit IgG (Dylight ® 488, ab98462) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with Alexa Fluor® 647-conjugated CD14 and rabbit IgG (Left) or ILT-4 (Right).
Data shown were gated on viable cells.
The CD14 co-staining result observed is consistent with what has been described in the literature (PMID:10879687).
ILT-4 is expressed in monocytes and CD14 is an established marker for monocytes and macrophages (PMID: 23382732).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224701).
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![Immunocytochemistry/ Immunofluorescence - Anti-ILT-4 antibody [EPR22081] - Low endotoxin, Azide free (ab246796)](https://www.abcam.com/ps/products/246/ab246796/Images/ab246796-1-ab2247011224701ICC1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ILT-4 antibody [EPR22081] - Low endotoxin, Azide free (ab246796)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Human PBMC (human primary peripheral blood mononuclear cell) cells labeling ILT-4 with ab224701 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing co-staining with CD14 and cytoplasmic staining in PBMC cells. The nuclear counterstain is DAPI (blue). Counterstained with an Alexa Fluor® 647 anti-human CD14 antibody at a 1/100 dilution (red).
The negative control is the secondary antibody only.
ILT-4 is expressed in monocytes (PMID:10879687) and CD14 is an established marker for monocytes and macrophages (PMID: 23382732).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224701).
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![Anti-ILT-4 antibody [EPR22081] - Low endotoxin, Azide free (ab246796)](https://www.abcam.com/ps/products/246/ab246796/Images/ab246796-3-benefits-of-recombinant-antibodies.png)
Anti-ILT-4 antibody [EPR22081] - Low endotoxin, Azide free (ab246796)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab246796 has not yet been referenced specifically in any publications.
