Recombinant Anti-ILT-4 antibody [EPR22081] - Low endotoxin, Azide free (ab246796)
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Overview
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Product name
Anti-ILT-4 antibody [EPR22081] - Low endotoxin, Azide free
See all ILT-4 primary antibodies -
Description
Rabbit monoclonal [EPR22081] to ILT-4 - Low endotoxin, Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human ILT-4 aa 1-250. The exact sequence is proprietary.
Database link: Q8N423 -
Positive control
- ICC/IF: Human PBMC. Flow Cyt: Human PBMC.
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General notes
ab246796 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Endotoxin level is less than 1 EU/ml as determined by the TAL test -
Clonality
Monoclonal -
Clone number
EPR22081 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab246796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. |
Target
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Function
Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance. Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. -
Tissue specificity
Expressed on monocytes and B-cells, and at lower levels on dendritic cells. Detected at low levels in natural killer (NK) cells. -
Sequence similarities
Contains 4 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
Contains 3 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Post-translational
modificationsPhosphorylated on tyrosine residues. Dephosphorylated by PTPN6. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10288 Human
- Omim: 604815 Human
- SwissProt: Q8N423 Human
- Unigene: 655652 Human
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Alternative names
- CD85 antigen-like family member D antibody
- CD85d antibody
- CD85d antigen antibody
see all
Images
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![Flow Cytometry - Anti-ILT-4 antibody [EPR22081] - Low endotoxin, Azide free (ab246796)](https://www.abcam.com/ps/products/246/ab246796/Images/ab246796-2-ab2247012224701FC1.jpg)
Flow Cytometry - Anti-ILT-4 antibody [EPR22081] - Low endotoxin, Azide free (ab246796)Flow cytometric analysis of human primary peripheral blood mononuclear cell (PBMC) labeling ILT-4 with ab224701 at 1/500 (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat anti rabbit IgG (Dylight ® 488, ab98462) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with Alexa Fluor® 647-conjugated CD14 and rabbit IgG (Left) or ILT-4 (Right).
Data shown were gated on viable cells.
The CD14 co-staining result observed is consistent with what has been described in the literature (PMID:10879687).
ILT-4 is expressed in monocytes and CD14 is an established marker for monocytes and macrophages (PMID: 23382732).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224701).
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![Immunocytochemistry/ Immunofluorescence - Anti-ILT-4 antibody [EPR22081] - Low endotoxin, Azide free (ab246796)](https://www.abcam.com/ps/products/246/ab246796/Images/ab246796-1-ab2247011224701ICC1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ILT-4 antibody [EPR22081] - Low endotoxin, Azide free (ab246796)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Human PBMC (human primary peripheral blood mononuclear cell) cells labeling ILT-4 with ab224701 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing co-staining with CD14 and cytoplasmic staining in PBMC cells. The nuclear counterstain is DAPI (blue). Counterstained with an Alexa Fluor® 647 anti-human CD14 antibody at a 1/100 dilution (red).
The negative control is the secondary antibody only.
ILT-4 is expressed in monocytes (PMID:10879687) and CD14 is an established marker for monocytes and macrophages (PMID: 23382732).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224701).
Datasheets and documents
References
ab246796 has not yet been referenced specifically in any publications.
