For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    immunoassay-blocking-bsa-free-ab171535.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Share by email

Immunoassay Blocking (BSA Free) (ab171535)

  • Datasheet
  • SDS
Submit a review Submit a question References (6)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

ELISA - Immunoassay Blocking (BSA Free) (ab171535)

    You may also be interested in

    Cocktail
    Product image
    Complex II WB Antibody Cocktail (ab110410)
    Primary
    Product image
    Anti-Cytochrome C antibody [EPR1327] (ab133504)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (Biotin) (ab207995)

    View more associated products

    Overview

    • Product name

      Immunoassay Blocking (BSA Free)
    • Tested applications

      Suitable for: ELISAmore details
      Unsuitable for: IHC-Fr or IHC-P
    • General notes

      Abcam’s Immunoassay Blocking (BSA Free) (ab171535) maintains the activity of antibodies and other biomolecules adsorbed or immobilized to solid substrates. It is easily incorporated into most assay protocols as the blocking solution, and minimal incubation is required. For blocking nonspecific sites on hydrophobic substrates, incubate for as little as five minutes and then proceed with your assay. Longer incubation times, while not necessary, are also not detrimental. For simultaneous blocking and stabilizing, apply ab171535 and incubate. Remove excess solution and completely dry the component. For maximum shelf life, store the stabilized component in a sealed container with desiccant.

      To Stabilize Adsorbed or Immobilized Proteins on Microtiter Plates/Strips
      1. For optimal stability and performance, use ab171535 at 100% concentration
      2. Immobilize or adsorb the primary protein (antibody or antigen) according to the procedure optimized in your laboratory; wash adequately to remove excess or weakly bound material
      3. Immediately after washing, add ab171535 to allow interaction with the entire protein-coated surface; do not let coated components dry before adding ab171535 since drying contributes to the loss of protein activity
      4. Incubate for five minutes to two hours at room temperature; for most assays, ab171535 can replace the blocking solution
      5. Remove or aspirate ab171535, but do not wash
      6. To dry components for long-term storage, we recommend one of the following methods:

      – Option 1. Place plates in a humidity-controlled chamber until dry (2-24 hours)
      – Option 2. Dry 2 -24 hours in a sealed desiccated container, such as a cabinet, jar, or plastic bag containing calcium sulfate or silica gel desiccant in amounts adequate for the size of the container, or
      – Option 3. Dry plates at 30°C-40°C in a vacuum oven for 2-4 hours

      NOTE: Drying times should be optimized for each application

      7. Immediately package the dried product in an airtight, moisture-proof container with a desiccant; this is especially important with the final product is stored in a humid environment or refrigerated where condensation is likely to occur

    Properties

    • Form

      Liquid
    • Storage instructions

      Store at room temperature.
    • Storage buffer

      pH: 7
      Constituent: 100% Proprietary component

      pH 6.6 - 7.2. Preservative: None.
    • Concentration information loading...

    Associated products

    • Related Products

      • Immunoassay Blocking Buffer (ab171534)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab171535 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA
    Use at an assay dependent concentration.
    Notes
    ELISA
    Use at an assay dependent concentration.
    Application notes
    Is unsuitable for IHC-Fr or IHC-P.

    Images

    • ELISA - Immunoassay Blocking (BSA Free) (ab171535)
      ELISA - Immunoassay Blocking (BSA Free) (ab171535)

      Stability tests were performed with Immunoassay Blocking (BSA Free) (ab171535) using either monoclonal or polyclonal IgG antibodies.

      Coated microtiter plates were stabilized with either ab171535 or 1% BSA. The antibody-coated plates were dried and placed at room temperature. Percent retained activity was determined by comparing the activity of the aged antibody-coated microtiter plates to that of freshly coated plates.

      After 30 months in Immunoassay Blocking (BSA Free) (ab171535) at room temperature, the monoclonal antibody retained greater than 85% activity and the polyclonal antibody retained 90% activity.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (6)

    Publishing research using ab171535? Please let us know so that we can cite the reference in this datasheet.

    ab171535 has been referenced in 6 publications.

    • Cao J  et al. Matrine has pro-apoptotic effects on liver cancer by triggering mitochondrial fission and activating Mst1-JNK signalling pathways. J Physiol Sci 69:185-198 (2019). PubMed: 30155612
    • Li J  et al. Liraglutide protects renal mesangial cells against hyperglycemia-mediated mitochondrial apoptosis by activating the ERK-Yap signaling pathway and upregulating Sirt3 expression. Mol Med Rep 19:2849-2860 (2019). PubMed: 30816450
    • Wang Y  et al. Sirt3-mediated mitochondrial fission regulates the colorectal cancer stress response by modulating the Akt/PTEN signalling pathway. Biomed Pharmacother 105:1172-1182 (2018). PubMed: 30021354
    • Ji K  et al. TAZ inhibition promotes IL-2-induced apoptosis of hepatocellular carcinoma cells by activating the JNK/F-actin/mitochondrial fission pathway. Cancer Cell Int 18:117 (2018). PubMed: 30127666
    • Wei R  et al. Matrine promotes liver cancer cell apoptosis by inhibiting mitophagy and PINK1/Parkin pathways. Cell Stress Chaperones 23:1295-1309 (2018). PubMed: 30209783
    • Bossi F  et al. Systematic discovery of novel eukaryotic transcriptional regulators using sequence homology independent prediction. BMC Genomics 18:480 (2017). PubMed: 28651538

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab171535.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.