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    imp3-antibody-ab176685.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Regulation
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Anti-IMP3 antibody (ab176685)

  • Datasheet
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Western blot - Anti-IMP3 antibody (ab176685)
  • Immunoprecipitation - Anti-IMP3 antibody (ab176685)

Key features and details

  • Rabbit polyclonal to IMP3
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-IMP3 antibody
    See all IMP3 primary antibodies
  • Description

    Rabbit polyclonal to IMP3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IMP3 aa 1-50. The exact sequence is proprietary.
    Sequence:

    MNKLYIGNLSENAAPSDLESIFKDAKIPVSGPFLVKTGYAFVDCPDESWA


    Database link: O00425
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa, Jurkat and 293T whole cell lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituent: 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab176685 was affinity purified using an epitope specific to IMP3 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Cancer
    • Tumor biomarkers
    • Hormones

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Jurkat whole cell lysate (ab7899)
  • Related Products

    • Recombinant Human IMP3 protein (denatured) (ab139801)

Applications

Our Abpromise guarantee covers the use of ab176685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 64 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA.
  • Tissue specificity

    Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines.
  • Sequence similarities

    Belongs to the RRM IMP/VICKZ family.
    Contains 4 KH domains.
    Contains 2 RRM (RNA recognition motif) domains.
  • Domain

    The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.
  • Cellular localization

    Nucleus. Cytoplasm. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Localized at the connecting piece and the tail of the spermatozoa. Colocalized with CD44 mRNA in RNP granules.
  • Target information above from: UniProt accession O00425 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10643 Human
    • Omim: 608259 Human
    • SwissProt: O00425 Human
    • Unigene: 700696 Human
    • Alternative names

      • Cancer/testis antigen 98 antibody
      • CT98 antibody
      • DKFZp686F1078 antibody
      • hKOC antibody
      • IF2B3_HUMAN antibody
      • IGF II mRNA binding protein 3 antibody
      • IGF-II mRNA-binding protein 3 antibody
      • IGF2 mRNA binding protein 3 antibody
      • IGF2 mRNA-binding protein 3 antibody
      • IGF2BP3 antibody
      • IMP 3 antibody
      • IMP-3 antibody
      • Insulin like growth factor 2 mRNA binding protein 3 antibody
      • Insulin-like growth factor 2 mRNA-binding protein 3 antibody
      • KH domain containing protein overexpressed in cancer antibody
      • KH domain-containing protein overexpressed in cancer antibody
      • KOC 1 antibody
      • KOC1 antibody
      • VICKZ 3 antibody
      • VICKZ family member 3 antibody
      • VICKZ3 antibody
      see all

    Images

    • Western blot - Anti-IMP3 antibody (ab176685)
      Western blot - Anti-IMP3 antibody (ab176685)
      All lanes : Anti-IMP3 antibody (ab176685) at 0.04 µg/ml

      Lane 1 : HeLa lysate at 50 µg
      Lane 2 : HeLa lysate at 15 µg
      Lane 3 : Jurkat lysate at 50 µg
      Lane 4 : 293T lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 64 kDa


      Exposure time: 3 minutes
    • Immunoprecipitation - Anti-IMP3 antibody (ab176685)
      Immunoprecipitation - Anti-IMP3 antibody (ab176685)

      Detection of Human IMP3 by Western Blot of Immunoprecipitates.

      Detection of IMP3 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176685 at 6 µg/mg lysate for IP. For WB analysis of immunoprecipitated IMP3, ab176685 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab176685? Please let us know so that we can cite the reference in this datasheet.

    ab176685 has been referenced in 1 publication.

    • Wu C  et al. Insulin-like growth factor II mRNA-binding protein 3 promotes cell proliferation, migration and invasion in human glioblastoma. Onco Targets Ther 12:3661-3670 (2019). PubMed: 31190868

    Customer reviews and Q&As

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