Overview

  • Product name
    Anti-IMP3 antibody [EPR12021]
    See all IMP3 primary antibodies
  • Description
    Rabbit monoclonal [EPR12021] to IMP3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human IMP3. The exact sequence is proprietary.
    Database link: O00425

  • Positive control
    • HeLa, BxPC-3 and 293T lysates; BxPC-3 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 69 kDa (predicted molecular weight: 63 kDa).
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA.
    • Tissue specificity
      Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines.
    • Sequence similarities
      Belongs to the RRM IMP/VICKZ family.
      Contains 4 KH domains.
      Contains 2 RRM (RNA recognition motif) domains.
    • Domain
      The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.
    • Cellular localization
      Nucleus. Cytoplasm. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Localized at the connecting piece and the tail of the spermatozoa. Colocalized with CD44 mRNA in RNP granules.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cancer/testis antigen 98 antibody
      • CT98 antibody
      • DKFZp686F1078 antibody
      • hKOC antibody
      • IF2B3_HUMAN antibody
      • IGF II mRNA binding protein 3 antibody
      • IGF-II mRNA-binding protein 3 antibody
      • IGF2 mRNA binding protein 3 antibody
      • IGF2 mRNA-binding protein 3 antibody
      • IGF2BP3 antibody
      • IMP 3 antibody
      • IMP-3 antibody
      • Insulin like growth factor 2 mRNA binding protein 3 antibody
      • Insulin-like growth factor 2 mRNA-binding protein 3 antibody
      • KH domain containing protein overexpressed in cancer antibody
      • KH domain-containing protein overexpressed in cancer antibody
      • KOC 1 antibody
      • KOC1 antibody
      • VICKZ 3 antibody
      • VICKZ family member 3 antibody
      • VICKZ3 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: IGF2BP3 (IMP3) knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: HEK293 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab177477 observed at 70 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab177477 was shown to specifically react with IGF2BP3 (IMP3) in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when IGF2BP3 (IMP3) knockout cells were examined. Wild-type and IGF2BP3 (IMP3) knockout samples were subjected to SDS-PAGE. Ab177477 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

    • Immunofluorescent analysis of BxPC-3 cells labeling IMP3 with ab177477 at 1/50 (green) and DAPI staining (blue).

    • Flow cytometry analysis of BxPC-3 (human pancreas adenocarcinoma) cells labeling with purified ab177477 at 1/300 dilution ( 10ug/ml) (Red). Cells were fixed with 80% methanol and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077) )(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as a isotype control.Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

    • All lanes : Anti-IMP3 antibody [EPR12021] (ab177477) at 1/1000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : BxPC-3 lysate
      Lane 3 : 293T lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 63 kDa

    • Immunoprecipitation of IMP3 from 293T cell lysate using ab177477 at 1/10.

    • All lanes : Anti-IMP3 antibody [EPR12021] (ab177477) at 1/20000 dilution

      Lane 1 : MDA-MB-231 (human breast adenocarcinoma) whole cell lysate
      Lane 2 : MDA-MB-435S (human melanoma) whole cell lysate
      Lane 3 : MCF-7 (human breast adenocarcinoma) whole cell lysate
      Lane 4 : Human Fetal Kidney

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/2000 dilution

      Predicted band size: 63 kDa
      Observed band size: 63 kDa



      Blocking/Diluting buffer 5% NFDM/TBST

      The protein expression of IMP-3 in MCF-7 cells is negative or undetectable (PMID: 22266872).

      Exposure time:
      Lane 1: 10 seconds
      Lane 2-4: 5 seconds

    References

    This product has been referenced in:
    • Lu L  et al. The expression of IMP3 in 366 cases with ovarian carcinoma of high grade serous, endometrioid and clear cell subtypes. Pathol Res Pract N/A:N/A (2018). IHC-P . Read more (PubMed: 29866423) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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