Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-IMP3 antibody [EPR12021] - BSA and Azide free (ab249982)

Overview

  • Product name

    Anti-IMP3 antibody [EPR12021] - BSA and Azide free
    See all IMP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR12021] to IMP3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IP, WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human IMP3. The exact sequence is proprietary.
    Database link: O00425

  • General notes

    Ab249982 is the carrier-free version of ab177477. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab249982 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab249982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 69 kDa (predicted molecular weight: 63 kDa).
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA.
    • Tissue specificity

      Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines.
    • Sequence similarities

      Belongs to the RRM IMP/VICKZ family.
      Contains 4 KH domains.
      Contains 2 RRM (RNA recognition motif) domains.
    • Domain

      The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.
    • Cellular localization

      Nucleus. Cytoplasm. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Localized at the connecting piece and the tail of the spermatozoa. Colocalized with CD44 mRNA in RNP granules.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cancer/testis antigen 98 antibody
      • CT98 antibody
      • DKFZp686F1078 antibody
      • hKOC antibody
      • IF2B3_HUMAN antibody
      • IGF II mRNA binding protein 3 antibody
      • IGF-II mRNA-binding protein 3 antibody
      • IGF2 mRNA binding protein 3 antibody
      • IGF2 mRNA-binding protein 3 antibody
      • IGF2BP3 antibody
      • IMP 3 antibody
      • IMP-3 antibody
      • Insulin like growth factor 2 mRNA binding protein 3 antibody
      • Insulin-like growth factor 2 mRNA-binding protein 3 antibody
      • KH domain containing protein overexpressed in cancer antibody
      • KH domain-containing protein overexpressed in cancer antibody
      • KOC 1 antibody
      • KOC1 antibody
      • VICKZ 3 antibody
      • VICKZ family member 3 antibody
      • VICKZ3 antibody
      see all

    References

    ab249982 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab249982.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up