Overview

  • Product name

    Anti-IMP3 antibody [EPR12021] (HRP)
    See all IMP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR12021] to IMP3 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human IMP3. The exact sequence is proprietary.
    Database link: O00425

  • Positive control

    • WB: HeLa, HEK293 cell lysates
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab208869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 63 kDa).

Target

  • Function

    RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA.
  • Tissue specificity

    Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines.
  • Sequence similarities

    Belongs to the RRM IMP/VICKZ family.
    Contains 4 KH domains.
    Contains 2 RRM (RNA recognition motif) domains.
  • Domain

    The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.
  • Cellular localization

    Nucleus. Cytoplasm. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Localized at the connecting piece and the tail of the spermatozoa. Colocalized with CD44 mRNA in RNP granules.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cancer/testis antigen 98 antibody
    • CT98 antibody
    • DKFZp686F1078 antibody
    • hKOC antibody
    • IF2B3_HUMAN antibody
    • IGF II mRNA binding protein 3 antibody
    • IGF-II mRNA-binding protein 3 antibody
    • IGF2 mRNA binding protein 3 antibody
    • IGF2 mRNA-binding protein 3 antibody
    • IGF2BP3 antibody
    • IMP 3 antibody
    • IMP-3 antibody
    • Insulin like growth factor 2 mRNA binding protein 3 antibody
    • Insulin-like growth factor 2 mRNA-binding protein 3 antibody
    • KH domain containing protein overexpressed in cancer antibody
    • KH domain-containing protein overexpressed in cancer antibody
    • KOC 1 antibody
    • KOC1 antibody
    • VICKZ 3 antibody
    • VICKZ family member 3 antibody
    • VICKZ3 antibody
    see all

Images

  • All lanes : Anti-IMP3 antibody [EPR12021] (HRP) (ab208869) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : IGF2BP3 (IMP3) knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 63 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    ab208869 was shown to specifically react with IMP3 in wild-type HAP1 cells as signal was lost in IGF2BP3 (IMP3) knockout cells. Wild-type and IGF2BP3 (IMP3) knockout samples were subjected to SDS-PAGE. Ab208869 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

  • All lanes : Anti-IMP3 antibody [EPR12021] (HRP) (ab208869) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 63 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab208869 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab208869 has not yet been referenced specifically in any publications.

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