Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-IMP3 antibody [EPR12022] (ab177942)

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Overview

  • Product name

    Anti-IMP3 antibody [EPR12022]
    See all IMP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR12022] to IMP3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Recombinant fragment within Human IMP3. The exact sequence is proprietary.
    Database link: O00425

  • Positive control

    • HeLa, 293T, K562 and HT-29 cell lysates; HeLa cells.

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab177942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 63 kDa).
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function

      RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA.

    • Tissue specificity

      Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines.

    • Sequence similarities

      Belongs to the RRM IMP/VICKZ family.
      Contains 4 KH domains.
      Contains 2 RRM (RNA recognition motif) domains.

    • Domain

      The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.

    • Cellular localization

      Nucleus. Cytoplasm. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Localized at the connecting piece and the tail of the spermatozoa. Colocalized with CD44 mRNA in RNP granules.
    • Information by UniProt

    • Database links

      • Alternative names

        • Cancer/testis antigen 98 antibody
        • CT98 antibody
        • DKFZp686F1078 antibody
        • hKOC antibody
        • IF2B3_HUMAN antibody
        • IGF II mRNA binding protein 3 antibody
        • IGF-II mRNA-binding protein 3 antibody
        • IGF2 mRNA binding protein 3 antibody
        • IGF2 mRNA-binding protein 3 antibody
        • IGF2BP3 antibody
        • IMP 3 antibody
        • IMP-3 antibody
        • Insulin like growth factor 2 mRNA binding protein 3 antibody
        • Insulin-like growth factor 2 mRNA-binding protein 3 antibody
        • KH domain containing protein overexpressed in cancer antibody
        • KH domain-containing protein overexpressed in cancer antibody
        • KOC 1 antibody
        • KOC1 antibody
        • VICKZ 3 antibody
        • VICKZ family member 3 antibody
        • VICKZ3 antibody
        see all

      Images

      • Western blot - Anti-IMP3 antibody [EPR12022] (ab177942)
        Western blot - Anti-IMP3 antibody [EPR12022] (ab177942)

        Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
        Lane 2: IGF2BP3 (IMP3) knockout HAP1 whole cell lysate (20 µg)
        Lane 3: HeLa whole cell lysate (20 µg)
        Lane 4: HEK293 whole cell lysate (20 µg)

        Lanes 1 - 4: Merged signal (red and green). Green - ab177942 observed at 70 kDa. Red - loading control, ab9484, observed at 37 kDa.

        ab177942 was shown to specifically react with IGF2BP3 (IMP3) in wild-type HAP1 cells as signal was lost in IGF2BP3 (IMP3) knockout cells. Wild-type and IGF2BP3 (IMP3) knockout samples were subjected to SDS-PAGE. Ab177942 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

      • Immunocytochemistry/ Immunofluorescence - Anti-IMP3 antibody [EPR12022] (ab177942)
        Immunocytochemistry/ Immunofluorescence - Anti-IMP3 antibody [EPR12022] (ab177942)

        Immunofluorescent analysis of HeLa cells labeling IMP3 with ab177942 at 1/50 dilution (green). DAPI nuclear staining (blue).

      • Immunoprecipitation - Anti-IMP3 antibody [EPR12022] (ab177942)
        Immunoprecipitation - Anti-IMP3 antibody [EPR12022] (ab177942)

        Western blot analysis on immunoprecipitation pellet from 293T cell lysate using ab177942.

      • Western blot - Anti-IMP3 antibody [EPR12022] (ab177942)
        Western blot - Anti-IMP3 antibody [EPR12022] (ab177942)
        All lanes : Anti-IMP3 antibody [EPR12022] (ab177942) at 1/1000 dilution

        Lane 1 : Hela cell lysate
        Lane 2 : 293T cell lysate
        Lane 3 : K562 cell lysate
        Lane 4 : HT-29 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 63 kDa
        Observed band size: 69 kDa
        why is the actual band size different from the predicted?

      References

      ab177942 has not yet been referenced specifically in any publications.

      Publishing research using ab177942? Please let us know so that we can cite the reference in this datasheet.

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