Overview

  • Product name

    Anti-IMPA1 antibody [EPR15404(B)]
    See all IMPA1 primary antibodies
  • Description

    Rabbit monoclonal [EPR15404(B)] to IMPA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human IMPA1 aa 50-150. The exact sequence is proprietary.
    Database link: P29218

  • Positive control

    • HCT116 and PC3 cells; Jurkat, PC3, HCT116 aand Human testis lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IP 1/40 - 1/60.
ICC/IF 1/100.

Target

  • Function

    Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Can use myo-inositol monophosphates, myo-inositol-1,3-diphosphate, myo-inositol-1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates.
  • Pathway

    Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.
  • Sequence similarities

    Belongs to the inositol monophosphatase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • IMP 1 antibody
    • IMP antibody
    • IMPA 1 antibody
    • IMPA antibody
    • IMPA1 antibody
    • IMPA1_HUMAN antibody
    • IMPase 1 antibody
    • IMPase antibody
    • Inositol 1(or 4) monophosphatase antibody
    • Inositol monophosphatase 1 antibody
    • Inositol monophosphatase antibody
    • Inositol(myo) 1(or 4) monophosphatase 1 antibody
    • Inositol-1(or 4)-monophosphatase 1 antibody
    • Lithium sensitive myo inositol monophosphatase A1 antibody
    • Lithium-sensitive myo-inositol monophosphatase A1 antibody
    see all

Images

  • All lanes : Anti-IMPA1 antibody [EPR15404(B)] (ab184165) at 1/50000 dilution

    Lane 1 : Human testis tissue lysate
    Lane 2 : Jurkat cell line lysate
    Lane 3 : PC 3 cell line lysate
    Lane 4 : HCT116 cell line lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 30 kDa

  • Immunofluorescent analysis of HCT116 cells (4% paraformaldehyde-fixed) labeling IMPA1 with ab184165 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Western blot analysis of immunoprecipitation pellet from Human testis tissue lysate (lane 1) or a PBS control (lane 2) immunoprecipitated using ab184165 at 1/50 dilution.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

     

  • Flow cytometric analysis of PC3 cells (2% paraformaldehyde-fixed) labeling IMPA1 with ab184165 at 1/40 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela)
Permeabilization
Yes - Triton 0.35%
Specification
Hela
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Methanol and PFA

Abcam user community

Verified customer

Submitted Sep 30 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (Hela cells)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
40 µg
Specification
Hela cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Sep 30 2015

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