Recombinant Anti-IMPA1 antibody [EPR15405] (ab191570)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15405] to IMPA1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-IMPA1 antibody [EPR15405]
See all IMPA1 primary antibodies -
Description
Rabbit monoclonal [EPR15405] to IMPA1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human testis lysate; Jurkat; PC3 and HCT 116 cell lysates; Mouse brain lysate; PC3 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15405 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/300.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/10000 - 1/100000. Predicted molecular weight: 30 kDa.
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Notes |
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Flow Cyt (Intra)
1/300. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/100000. Predicted molecular weight: 30 kDa. |
Target
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Function
Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Can use myo-inositol monophosphates, myo-inositol-1,3-diphosphate, myo-inositol-1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates. -
Pathway
Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2. -
Sequence similarities
Belongs to the inositol monophosphatase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3612 Human
- Entrez Gene: 55980 Mouse
- Entrez Gene: 83523 Rat
- Omim: 602064 Human
- SwissProt: P29218 Human
- SwissProt: O55023 Mouse
- SwissProt: P97697 Rat
- Unigene: 656694 Human
see all -
Alternative names
- IMP 1 antibody
- IMP antibody
- IMPA 1 antibody
see all
Images
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Intracellular flow cytometric analysis of PC3 cells labeling IMPA1 using ab191570at 1/300 dilution (red), Isotype control (green). Secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. Cells were fixed in 2% paraformaldehyde prior to staining.
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Anti-IMPA1 antibody [EPR15405] (ab191570) at 1/20000 dilution + Mouse brain tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 30 kDa5% NFDM/TBST blocking buffer
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All lanes : Anti-IMPA1 antibody [EPR15405] (ab191570) at 1/20000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : PC3 cell lysate
Lane 3 : HCT 116 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 30 kDa5% NFDM/TBST blocking buffer
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Anti-IMPA1 antibody [EPR15405] (ab191570) at 1/100000 dilution + Human testis lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 30 kDa5% NFDM/TBST blocking buffer
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab191570 has not yet been referenced specifically in any publications.